Histochemical identification of cultured cells from human endometrium

Jill M. Siegfried, Karen G. Nelson, Jane L. Martin, David G. Kaufman

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

Histochemical techniques have been applied to the identification of cell types cultured from human endometrium. Previous work from this laboratory characterized two principtal cell types found in cultures of endometrium: a mature epithelial cell and another cell which was classified as the endometrial stromal cell based on light and electron microscopy. In this report we compare the histochemical staining of endometrial tissue in frozen sections to that of cultured cells. These results confirm the epithelial and stromal nature of the respective cell types. Several markers were found that could distinguish between cells of epithelial and stromal origin. The enzymes alkaline phosphatase, γ-glutamyltranspeptidase, peroxidase, and β-glucuronidase were localized in glandular and surface epithelia in frozen sections and in colonies of epithelial cells in culture. Stroma in frozen sections and cultured stromal cells contained leucine aminopeptidase and fibronectin. Epithelial sections and in culture could also be distinguished from cells of stromal origin by preferential binding of lotus and peanut lectin. Several other markers were found in both endometrial epithelium and stroma.

Original languageEnglish (US)
Pages (from-to)25-32
Number of pages8
JournalIn Vitro: Journal of the Tissue Culture Association
Volume20
Issue number1
DOIs
StatePublished - Jan 1984

Keywords

  • endometrium
  • histochemistry
  • human cell culture

Fingerprint Dive into the research topics of 'Histochemical identification of cultured cells from human endometrium'. Together they form a unique fingerprint.

Cite this