Highly resolved in vivo 1H NMR spectroscopy of the mouse brain at 9.4 T

Ivan Tkáč, Pierre Gilles Henry, Peter Andersen, C. Dirk Keene, Walter C. Low, Rolf Gruetter

Research output: Contribution to journalArticlepeer-review

155 Scopus citations

Abstract

An efficient shim system and an optimized localization sequence were used to measure in vivo 1H NMR spectra from cerebral cortex, hippocampus, striatum, and cerebellum of C57BL/6 mice at 9.4 T. The combination of automatic first- and second-order shimming (FASTMAP) with strong custom-designed second-order shim coils (shim strength up to 0.04 mT/cm2) was crucial to achieve high spectral resolution (water line width of 11-14 Hz). Requirements for second-order shim strengths to compensate field inhomogeneities in the mouse brain at 9.4 T were assessed. The achieved spectral quality (resolution, S/N, water suppression, localization performance) allowed reliable quantification of 16 brain metabolites (LCModel analysis) from 5-10-μL brain volumes. Significant regional differences (up to 2-fold, P < 0.05) were found for all quantified metabolites but Asp, Glc, and Gln. In contrast, 1H NMR spectra measured from the striatum of C57BL/6, CBA, and CBA/BL6 mice revealed only small (<13%, P < 0.05) interstrain differences in Gln, Glu, Ins, Lac, HAAG, and PE. It is concluded that 1H NMR spectroscopy at 9.4 T can provide precise biochemical information from distinct regions of the mouse brain noninvasively that can be used for monitoring of disease progression and treatment as well as phenotyping in transgenic mice models.

Original languageEnglish (US)
Pages (from-to)478-484
Number of pages7
JournalMagnetic resonance in medicine
Volume52
Issue number3
DOIs
StatePublished - Sep 2004

Keywords

  • In vivo NMR spectroscopy
  • Mouse brain
  • Neurochemical profile
  • Quantification
  • Shimming

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