In vitro selection using mRNA display is currently a widely used method to isolate functional peptides with desired properties. The analysis of high throughput sequencing (HTS) data from in vitro evolution experiments has proven to be a powerful technique but only recently has it been applied to mRNA display selections. In this Perspective, we introduce aspects of mRNA display and HTS that may be of interest to physical chemists. We highlight the potential of HTS to analyze in vitro selections of peptides and review recent advances in the application of HTS analysis to mRNA display experiments. We discuss some possible issues involved with HTS analysis and summarize some strategies to alleviate them. Finally, the potential for future impact of advancing HTS analysis on mRNA display experiments is discussed.
Bibliographical noteFunding Information:
The authors thank the Otis Williams Fund, the Simons Collaboration on the Origins of Life (290356FY18 and 340762), the US National Institutes of Health (GM108703), the US National Aeronautics and Space Administration (NNX14AK29G, NNX16AJ32G) and the NIH New Innovator Program (DP2GM123457) for support.
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