High throughput sequencing of: In vitro selections of mRNA-displayed peptides: Data analysis and applications

Celia Blanco, Samuel Verbanic, Burckhard Seelig, Irene A. Chen

Research output: Contribution to journalReview article

1 Scopus citations

Abstract

In vitro selection using mRNA display is currently a widely used method to isolate functional peptides with desired properties. The analysis of high throughput sequencing (HTS) data from in vitro evolution experiments has proven to be a powerful technique but only recently has it been applied to mRNA display selections. In this Perspective, we introduce aspects of mRNA display and HTS that may be of interest to physical chemists. We highlight the potential of HTS to analyze in vitro selections of peptides and review recent advances in the application of HTS analysis to mRNA display experiments. We discuss some possible issues involved with HTS analysis and summarize some strategies to alleviate them. Finally, the potential for future impact of advancing HTS analysis on mRNA display experiments is discussed.

Original languageEnglish (US)
Pages (from-to)6492-6506
Number of pages15
JournalPhysical Chemistry Chemical Physics
Volume22
Issue number12
DOIs
StatePublished - Mar 28 2020

PubMed: MeSH publication types

  • Journal Article
  • Review

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