Recent studies have proven that skeleton-wide functional assessment is essential to comprehensively understand physiological aspects of the skeletal system. Therefore, in contrast to regional imaging studies utilizing a multiple-animal holder (mouse hotel), we attempted to develop and characterize a multiple-mouse imaging system with micro-PET/CT for high-throughput whole-skeleton assessment. Using items found in a laboratory, a simple mouse hotel that houses four mice linked with gas anesthesia was constructed. A mouse-simulating phantom was used to measure uniformity in a cross sectional area and flatness (Amax/Amin*100) along the axial, radial and tangential directions, where Amax and Amin are maximum and minimum activity concentration in the profile, respectively. Fourteen mice were used for single- or multiple-micro-PET/CT scans. NaF uptake was measured at eight skeletal sites (skull to tibia). Skeletal 18F activities measured with mice in the mouse hotel were within 1.6±4% (mean±standard deviation) of those measured with mice in the single-mouse holder. Single-holder scanning yields slightly better uniformity and flatness over the hotel. Compared to use of the single-mouse holder, scanning with the mouse hotel reduced study time (by 65%), decreased the number of scans (four-fold), reduced cost, required less computer storage space (40%), and maximized 18F usage. The mouse hotel allows high-throughput, quantitatively equivalent scanning compared to the single-mouse holder for micro-PET/CT imaging for whole-skeleton assessment of mice.
Bibliographical noteFunding Information:
This work was supported by the National Institutes of Health (1R01CA154491-01, 1R03AR055333-01A1 and 1K12-HD055887-01) and Japan Society for the Promotion of Science Core to Core Program (23003).
This work was supported by the National Institutes of Health ( 1R01CA154491-01 , 1R03AR055333-01A1 and 1K12-HD055887-01 ) and Japan Society for the Promotion of Science Core to Core Program ( 23003 ). The authors acknowledge and thank Dr. Kihak Lee (Siemens Medical Solutions, USA) for advice and fruitful discussion pertaining to this experiment and report and Timothy C. Doyle for providing information on potential mouse holder system.
- Mouse hotel
- Multiple-animal holder