TY - JOUR
T1 - High-throughput genomic sequencing of cassava bacterial blight strains identifies conserved effectors to target for durable resistance
AU - Bart, Rebecca
AU - Cohn, Megan
AU - Kassen, Andrew
AU - McCallum, Emily J.
AU - Shybut, Mikel
AU - Petriello, Annalise
AU - Krasileva, Ksenia
AU - Dahlbeck, Douglas
AU - Medina, Cesar
AU - Alicai, Titus
AU - Kumar, Lava
AU - Moreira, Leandro M.
AU - Neto, Júlio Rodrigues
AU - Verdier, Valerie
AU - Santana, María Angélica
AU - Kositcharoenkul, Nuttima
AU - Vanderschuren, Hervé
AU - Gruissem, Wilhelm
AU - Bernal, Adriana
AU - Staskawicz, Brian J.
PY - 2012/7/10
Y1 - 2012/7/10
N2 - Cassava bacterial blight (CBB), incited by Xanthomonas axonopodis pv. manihotis (Xam), is the most important bacterial disease of cassava, a staple food source for millions of people in developing countries. Here we present a widely applicable strategy for elucidating the virulence components of a pathogen population. We report Illumina-based draft genomes for 65 Xam strains and deduce the phylogenetic relatedness of Xam across the areas where cassava is grown. Using an extensive database of effector proteins from animal and plant pathogens, we identify the effector repertoire for each sequenced strain and use a comparative sequence analysis to deduce the least polymorphic of the conserved effectors. These highly conserved effectors have been maintained over 11 countries, three continents, and 70 y of evolution and as such represent ideal targets for developing resistance strategies.
AB - Cassava bacterial blight (CBB), incited by Xanthomonas axonopodis pv. manihotis (Xam), is the most important bacterial disease of cassava, a staple food source for millions of people in developing countries. Here we present a widely applicable strategy for elucidating the virulence components of a pathogen population. We report Illumina-based draft genomes for 65 Xam strains and deduce the phylogenetic relatedness of Xam across the areas where cassava is grown. Using an extensive database of effector proteins from animal and plant pathogens, we identify the effector repertoire for each sequenced strain and use a comparative sequence analysis to deduce the least polymorphic of the conserved effectors. These highly conserved effectors have been maintained over 11 countries, three continents, and 70 y of evolution and as such represent ideal targets for developing resistance strategies.
KW - Innate immunity
KW - Next-generation sequencing
KW - Type three effectors
UR - https://www.scopus.com/pages/publications/84863952738
UR - https://www.scopus.com/inward/citedby.url?scp=84863952738&partnerID=8YFLogxK
U2 - 10.1073/pnas.1208003109
DO - 10.1073/pnas.1208003109
M3 - Article
C2 - 22699502
AN - SCOPUS:84863952738
SN - 0027-8424
VL - 109
SP - E1972-E1979
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 28
ER -