High-throughput genomic sequencing of cassava bacterial blight strains identifies conserved effectors to target for durable resistance

Rebecca Bart, Megan Cohn, Andrew Kassen, Emily J. McCallum, Mikel Shybut, Annalise Petriello, Ksenia Krasileva, Douglas Dahlbeck, Cesar Medina, Titus Alicai, Lava Kumar, Leandro M. Moreira, Júlio Rodrigues Neto, Valerie Verdier, María Angélica Santana, Nuttima Kositcharoenkul, Hervé Vanderschuren, Wilhelm Gruissem, Adriana Bernal, Brian J. Staskawicz

Research output: Contribution to journalArticlepeer-review

108 Scopus citations

Abstract

Cassava bacterial blight (CBB), incited by Xanthomonas axonopodis pv. manihotis (Xam), is the most important bacterial disease of cassava, a staple food source for millions of people in developing countries. Here we present a widely applicable strategy for elucidating the virulence components of a pathogen population. We report Illumina-based draft genomes for 65 Xam strains and deduce the phylogenetic relatedness of Xam across the areas where cassava is grown. Using an extensive database of effector proteins from animal and plant pathogens, we identify the effector repertoire for each sequenced strain and use a comparative sequence analysis to deduce the least polymorphic of the conserved effectors. These highly conserved effectors have been maintained over 11 countries, three continents, and 70 y of evolution and as such represent ideal targets for developing resistance strategies.

Original languageEnglish (US)
Pages (from-to)E1972-E1979
JournalProceedings of the National Academy of Sciences of the United States of America
Volume109
Issue number28
DOIs
StatePublished - Jul 10 2012
Externally publishedYes

Keywords

  • Innate immunity
  • Next-generation sequencing
  • Type three effectors

Fingerprint

Dive into the research topics of 'High-throughput genomic sequencing of cassava bacterial blight strains identifies conserved effectors to target for durable resistance'. Together they form a unique fingerprint.

Cite this