High throughput 3D super-resolution microscopy reveals Caulobacter crescentus in vivo Z-ring organization

Seamus J. Holden, Thomas Pengo, Karin L. Meibom, Carmen Fernandez Fernandez, Justine Collier, Suliana Manley

Research output: Contribution to journalArticlepeer-review

133 Scopus citations

Abstract

We created a high-throughput modality of photoactivated localization microscopy (PALM) that enables automated 3D PALM imaging of hundreds of synchronized bacteria during all stages of the cell cycle. We used high-throughput PALM to investigate the nanoscale organization of the bacterial cell division protein FtsZ in live Caulobacter crescentus. We observed that FtsZ predominantly localizes as a patchy midcell band, and only rarely as a continuous ring, supporting a model of "Z-ring" organization whereby FtsZ protofilaments are randomly distributed within the band and interact only weakly. We found evidence for a previously unidentified period of rapid ring contraction in the final stages of the cell cycle. We also found that DNA damage resulted in production of high-density continuous Z-rings, which may obstruct cytokinesis. Our results provide a detailed quantitative picture of in vivo Z-ring organization.

Original languageEnglish (US)
Pages (from-to)4566-4571
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume111
Issue number12
DOIs
StatePublished - Mar 25 2014

Bibliographical note

Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.

Keywords

  • Bacterial cytoskeleton
  • SOS response

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