High-speed liquid chromatographic analysis of prostaglandins in rat kidney

Earl W Dunham, M. W. Anders

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Conditions for the measurement of prostaglandin B by high-speed liquid chromatography with ultra violet detection are described. The method is simple, rapid and can detect as little as 5 ng of prostaglandin B2. Prostaglandins E and A were analyzed in lipid extracts of incubated rat renal papilla homogenates as prostaglandin B following treatment with base. The values for prostaglandin synthesis obtained with the high-speed liquid chromatographic method compared favorably with results obtained by bioassay.

Original languageEnglish (US)
Pages (from-to)85-92
Number of pages8
JournalProstaglandins
Volume4
Issue number1
DOIs
StatePublished - Jul 1973

Bibliographical note

Funding Information:
ACKNOWLEDGEMENTS: Supported in part by the Minnesota Heart Association and the USPHS Grant No. HL 15447 and GM 15477. We thank Dr. John E. Pike of The Upjohn Co. for the generous gift of prostaglandins and Teresita Loy for skillful technical assistance.

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