High-resolution structure of the open NaK channel

Amer Alam, Youxing Jiang

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112 Scopus citations


We report the crystal structure of the nonselective cation channel NaK from Bacillus cereus at a resolution of 1.6 Å. The structure reveals the intracellular gate in an open state, as opposed to the closed form reported previously, making NaK the only channel for which the three-dimensional structures of both conformations are known. Channel opening follows a conserved mechanism of inner helix bending using a flexible glycine residue, the gating hinge, seen in MthK and most other tetrameric cation channels. Additionally, distinct inter and intrasubunit rearrangements involved in channel gating are seen and characterized for the first time along with inner helix twisting motions. Furthermore, we identify a residue deeper within the cavity of the channel pore, Phe92, which is likely to form a constriction point within the open pore, restricting ion flux through the channel. Mutating this residue to alanine causes a subsequent increase in ion-conduction rates as measured by 86Rb flux assays. The structures of both the open and closed conformations of the NaK channel correlate well with those of equivalent K + channel conformations, namely MthK and KcsA, respectively.

Original languageEnglish (US)
Pages (from-to)30-34
Number of pages5
JournalNature Structural and Molecular Biology
Issue number1
StatePublished - Jan 2009
Externally publishedYes

Bibliographical note

Funding Information:
Use of the Argonne National Laboratory Structural Biology Center beamlines at the Advanced Photon Source was supported by the US Department of Energy, Office of Energy Research. We thank the beamline staff for assistance in data collection. This work was supported by the Howard Hughes Medical Institute and by grants from the the US National Institutes of Health and National Institute of General Medical Sciences (RO1 GM079179), the David and Lucile Packard Foundation and the McKnight Endowment for Neuroscience. A.A. was supported by National Institutes of Health Training Grant T32 GM008297.


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