TY - JOUR
T1 - High-resolution radiation hybrid map of wheat chromosome 1D
AU - Kalavacharla, Venu
AU - Hossain, Khwaja
AU - Gu, Yong
AU - Riera-Lizarazu, Oscar
AU - Vales, M. Isabel
AU - Bhamidimarri, Suresh
AU - Gonzalez-Hernandez, Jose L.
AU - Maan, Shivcharan S.
AU - Kianian, Shahryar F.
PY - 2006
Y1 - 2006
N2 - Physical mapping methods that do not rely on meiotic recombination are necessary for complex polyploid genomes such as wheat (Triticum aestivum L.). This need is due to the uneven distribution of recombination and significant variation in genetic to physical distance ratios. One method that has proven valuable in a number of nonplant and plant systems is radiation hybrid (RH) mapping. This work presents, for the first time, a high-resolution radiation hybrid map of wheat chromosome 1D (D genome) in a tetraploid durum wheat (T. turgidum L., AB genomes) background. An RH panel of 87 lines was used to map 378 molecular markers, which detected 2312 chromosome breaks. The total map distance ranged from ∼3,341 cR35,000 for five major linkage groups to 11,773 cR35,000 for a comprehensive map. The mapping resolution was estimated to be ∼199 kb/break and provided the starting point for BAC contig alignment. To date, this is the highest resolution that has been obtained by plant RH mapping and serves as a first step for the development of RH resources in wheat.
AB - Physical mapping methods that do not rely on meiotic recombination are necessary for complex polyploid genomes such as wheat (Triticum aestivum L.). This need is due to the uneven distribution of recombination and significant variation in genetic to physical distance ratios. One method that has proven valuable in a number of nonplant and plant systems is radiation hybrid (RH) mapping. This work presents, for the first time, a high-resolution radiation hybrid map of wheat chromosome 1D (D genome) in a tetraploid durum wheat (T. turgidum L., AB genomes) background. An RH panel of 87 lines was used to map 378 molecular markers, which detected 2312 chromosome breaks. The total map distance ranged from ∼3,341 cR35,000 for five major linkage groups to 11,773 cR35,000 for a comprehensive map. The mapping resolution was estimated to be ∼199 kb/break and provided the starting point for BAC contig alignment. To date, this is the highest resolution that has been obtained by plant RH mapping and serves as a first step for the development of RH resources in wheat.
UR - http://www.scopus.com/inward/record.url?scp=33745377553&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33745377553&partnerID=8YFLogxK
U2 - 10.1534/genetics.106.056481
DO - 10.1534/genetics.106.056481
M3 - Article
C2 - 16624903
AN - SCOPUS:33745377553
VL - 173
SP - 1089
EP - 1099
JO - Genetics
JF - Genetics
SN - 0016-6731
IS - 2
ER -