High resolution proton NMR studies of perfused rat hearts

Kamil Ugurbil, Marc Petein, Rubin Maidan, Steve Michurski, Jay N. Cohn, Arthur H. From

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66 Scopus citations

Abstract

High resolution 1H NMR spectra of perfused rat hearts have been obtained under normoxic, ischemic and hypoxic conditions. Several myocardial metabolites including taurine, carnitine, lactate and tissue glycerides are detected in the 1H NMR spectra. Changes in oxygen availability induce perturbations in the levels of some metabolites, in particular, lactate. Experiments with fasted rats and with substrate-free perfusion suggest that the glycerides detected in 1H spectra are metabolically mobilizable but have a slow rate of turnover. These results demonstrate that utility of 1H NMR in monitoring myocardial metabolism.

Original languageEnglish (US)
Pages (from-to)73-78
Number of pages6
JournalFEBS Letters
Volume167
Issue number1
DOIs
StatePublished - Feb 13 1984

Bibliographical note

Funding Information:
This work was supportedi n part by grant HL 17871f rom the National Heart, Lung and Blood Institute, Veterans Admi~stration Medical Research Funds, and a grant from Dr Carl ThomaeG mbh, Biberacha n der Riss (FRG). K. U. is the recipient of an NIH Career Development Award.

Keywords

  • H-NMR
  • Myocardial metabolism
  • Perfused rat heart

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