High resolution proton NMR studies of perfused rat hearts

Kamil Ugurbil; Marc Petein; Rubin Maidan; Steve Michurski; Jay N. Cohn; Arthur H. From

doi:10.1016/0014-5793(84)80835-2

High resolution proton NMR studies of perfused rat hearts

Kamil Ugurbil, Marc Petein, Rubin Maidan, Steve Michurski, Jay N. Cohn, Arthur H. From

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Abstract

High resolution ¹H NMR spectra of perfused rat hearts have been obtained under normoxic, ischemic and hypoxic conditions. Several myocardial metabolites including taurine, carnitine, lactate and tissue glycerides are detected in the ¹H NMR spectra. Changes in oxygen availability induce perturbations in the levels of some metabolites, in particular, lactate. Experiments with fasted rats and with substrate-free perfusion suggest that the glycerides detected in ¹H spectra are metabolically mobilizable but have a slow rate of turnover. These results demonstrate that utility of ¹H NMR in monitoring myocardial metabolism.

Original language	English (US)
Pages (from-to)	73-78
Number of pages	6
Journal	FEBS Letters
Volume	167
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(84)80835-2
State	Published - Feb 13 1984

Bibliographical note

Funding Information:
This work was supportedi n part by grant HL 17871f rom the National Heart, Lung and Blood Institute, Veterans Admi~stration Medical Research Funds, and a grant from Dr Carl ThomaeG mbh, Biberacha n der Riss (FRG). K. U. is the recipient of an NIH Career Development Award.

Keywords

H-NMR
Myocardial metabolism
Perfused rat heart

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10.1016/0014-5793(84)80835-2

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title = "High resolution proton NMR studies of perfused rat hearts",

abstract = "High resolution 1H NMR spectra of perfused rat hearts have been obtained under normoxic, ischemic and hypoxic conditions. Several myocardial metabolites including taurine, carnitine, lactate and tissue glycerides are detected in the 1H NMR spectra. Changes in oxygen availability induce perturbations in the levels of some metabolites, in particular, lactate. Experiments with fasted rats and with substrate-free perfusion suggest that the glycerides detected in 1H spectra are metabolically mobilizable but have a slow rate of turnover. These results demonstrate that utility of 1H NMR in monitoring myocardial metabolism.",

keywords = "H-NMR, Myocardial metabolism, Perfused rat heart",

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note = "Funding Information: This work was supportedi n part by grant HL 17871f rom the National Heart, Lung and Blood Institute, Veterans Admi~stration Medical Research Funds, and a grant from Dr Carl ThomaeG mbh, Biberacha n der Riss (FRG). K. U. is the recipient of an NIH Career Development Award.",

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doi = "10.1016/0014-5793(84)80835-2",

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T1 - High resolution proton NMR studies of perfused rat hearts

AU - Ugurbil, Kamil

AU - Petein, Marc

AU - Maidan, Rubin

AU - Michurski, Steve

AU - Cohn, Jay N.

AU - From, Arthur H.

N1 - Funding Information: This work was supportedi n part by grant HL 17871f rom the National Heart, Lung and Blood Institute, Veterans Admi~stration Medical Research Funds, and a grant from Dr Carl ThomaeG mbh, Biberacha n der Riss (FRG). K. U. is the recipient of an NIH Career Development Award.

PY - 1984/2/13

Y1 - 1984/2/13

N2 - High resolution 1H NMR spectra of perfused rat hearts have been obtained under normoxic, ischemic and hypoxic conditions. Several myocardial metabolites including taurine, carnitine, lactate and tissue glycerides are detected in the 1H NMR spectra. Changes in oxygen availability induce perturbations in the levels of some metabolites, in particular, lactate. Experiments with fasted rats and with substrate-free perfusion suggest that the glycerides detected in 1H spectra are metabolically mobilizable but have a slow rate of turnover. These results demonstrate that utility of 1H NMR in monitoring myocardial metabolism.

AB - High resolution 1H NMR spectra of perfused rat hearts have been obtained under normoxic, ischemic and hypoxic conditions. Several myocardial metabolites including taurine, carnitine, lactate and tissue glycerides are detected in the 1H NMR spectra. Changes in oxygen availability induce perturbations in the levels of some metabolites, in particular, lactate. Experiments with fasted rats and with substrate-free perfusion suggest that the glycerides detected in 1H spectra are metabolically mobilizable but have a slow rate of turnover. These results demonstrate that utility of 1H NMR in monitoring myocardial metabolism.

KW - H-NMR

KW - Myocardial metabolism

KW - Perfused rat heart

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High resolution proton NMR studies of perfused rat hearts

Abstract

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