High-purity neutrophil isolation from human peripheral blood and saliva for transcriptome analysis

Flavia S. Lakschevitz, Michael Glogauer

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Scopus citations

Abstract

The oral cavity is a source of readily available neutrophils and can be used as a model to better understand the role of neutrophils in chronic inflammatory diseases such as rheumatoid arthritis, bronchitis, periodontitis, and inflammatory bowel disease. In this chapter we describe reproducible methods to obtain highly purified neutrophil samples from blood and saliva in humans to enable cell analysis using whole-genome microarrays.

Original languageEnglish (US)
Title of host publicationNeutrophil Methods and Protocols
PublisherHumana Press Inc.
Pages469-483
Number of pages15
ISBN (Print)9781627038447
DOIs
StatePublished - 2014
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1124
ISSN (Print)1064-3745

Keywords

  • Microarray
  • Oral neutrophils
  • Saliva
  • Transcriptome analysis

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