High Pressure Liquid Chromatographic Determination of Hydrochlorothiazide in Human Serum and Urine

M. J. Cooper, Alan R Sinaiko, M. W. Anders, B. L. Mirkin

Research output: Contribution to journalArticle

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Abstract

A new method has been developed for the quantitative estimation of the diuretic and antihypertensive agent, hydrochlorothiazide, in human serum and urine. The drug is extracted into ethyl acetate from biological material and then back-extracted into sodium hydroxide solution. This aqueous solution is chromatographed on a reverse phase system, using a µBondapak C18 column and methanol–0.01 M sodium dihydrogen phosphate as eluent and detection at 271 nm. The recovery from serum was 98.2 ± 3.7% (mean ± SE, n= 9) over the concentration range of 200-800 ng/ml. In urine, the recovery was 91.5 ± 2.5% (mean ± SE, n= 8) over the range of 20-100 µg/ml. No interference was observed in this assay from the following drugs which may be administered concurrently during hydrochlorothiazide therapy: azathloprine, chlorthalidone, guanethidine, methyldopa, minoxidil, prednisone, and spironolactone.

Original languageEnglish (US)
Pages (from-to)1110-1111
Number of pages2
JournalAnalytical chemistry
Volume48
Issue number8
DOIs
StatePublished - Jul 1 1976

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Hydrochlorothiazide
Chlorthalidone
Minoxidil
Methyldopa
Guanethidine
Recovery
Sodium Hydroxide
Spironolactone
Liquids
Prednisone
Diuretics
Pharmaceutical Preparations
Biological materials
Antihypertensive Agents
Assays
sodium phosphate
ethyl acetate

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High Pressure Liquid Chromatographic Determination of Hydrochlorothiazide in Human Serum and Urine. / Cooper, M. J.; Sinaiko, Alan R; Anders, M. W.; Mirkin, B. L.

In: Analytical chemistry, Vol. 48, No. 8, 01.07.1976, p. 1110-1111.

Research output: Contribution to journalArticle

Cooper, M. J. ; Sinaiko, Alan R ; Anders, M. W. ; Mirkin, B. L. / High Pressure Liquid Chromatographic Determination of Hydrochlorothiazide in Human Serum and Urine. In: Analytical chemistry. 1976 ; Vol. 48, No. 8. pp. 1110-1111.
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