High polymorphism level of genomic sequences flanking insertion sites of human endogenous retroviral long terminal repeats

Irina Lavrentieva, Natalia E. Broude, Yuri Lebedev, Irving I. Gottesman, Sergei A. Lukyanov, Cassandra L. Smith, Eugene D. Sverdlov

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32 Scopus citations

Abstract

The polymorphism at the multitude of loci adjacent to human endogenous retrovirus long terminal repeats (LTRs) was analyzed by a technique for whole genome differential display based on the PCR suppression effect that provides selective amplification and display of genomic sequences flanking interspersed repeated elements. This strategy is simple, target-specific, requires a small amount of DNA and provides reproducible and highly informative data. The average frequency of polymorphism observed in the vicinity of the LTR insertion sites was found to be about 12%. The high incidence of polymorphism within the LTR flanks together with the frequent location of LTRs near genes makes the LTR loci a useful source of polymorphic markers for gene mapping. Copyright (C) 1999 Federation of European Biochemical Societies.

Original languageEnglish (US)
Pages (from-to)341-347
Number of pages7
JournalFEBS Letters
Volume443
Issue number3
DOIs
StatePublished - Jan 29 1999

Keywords

  • Genomic differential display
  • Monozygotic twins
  • Polymorphism
  • Suppression polymerase chain reaction

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    Lavrentieva, I., Broude, N. E., Lebedev, Y., Gottesman, I. I., Lukyanov, S. A., Smith, C. L., & Sverdlov, E. D. (1999). High polymorphism level of genomic sequences flanking insertion sites of human endogenous retroviral long terminal repeats. FEBS Letters, 443(3), 341-347. https://doi.org/10.1016/S0014-5793(99)00004-6