Abstract
A reverse-phase high-performance liquid chromatographic technique with isocratic elution has been developed to separate and quantitate the major phenolic compounds of the hydroalcoholic extracts of olive oils. Hydroxytyrosol, tyrosol, caffeic acid, p-hydroxyphenylacetic acid and homovanillic acid were analyzed on a μBonapak C18 column with an acetonitrile/water/acetic acid (20:90:0.18, vol/vol/vol) mixture as a mobile phase. Electrochemical detection provided selectivity as well as sensitivity. The method was applied to the analysis of the most important phenolic compounds in olive oils.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 367-370 |
| Number of pages | 4 |
| Journal | Journal of the American Oil Chemists' Society |
| Volume | 70 |
| Issue number | 4 |
| DOIs | |
| State | Published - Apr 1993 |
Keywords
- Caffeic acid
- HPLC
- electrochemical detection
- homovanillic acid
- hydroxytyrosol
- olive oil
- p-hydroxyphenylacetic acid
- tyrosol