High-performance liquid chromatography of selected phenolic compounds in olive oils

M. Akasbi, D. W. Shoeman, A. Saari Csallany

Research output: Contribution to journalArticlepeer-review

64 Scopus citations


A reverse-phase high-performance liquid chromatographic technique with isocratic elution has been developed to separate and quantitate the major phenolic compounds of the hydroalcoholic extracts of olive oils. Hydroxytyrosol, tyrosol, caffeic acid, p-hydroxyphenylacetic acid and homovanillic acid were analyzed on a μBonapak C18 column with an acetonitrile/water/acetic acid (20:90:0.18, vol/vol/vol) mixture as a mobile phase. Electrochemical detection provided selectivity as well as sensitivity. The method was applied to the analysis of the most important phenolic compounds in olive oils.

Original languageEnglish (US)
Pages (from-to)367-370
Number of pages4
JournalJournal of the American Oil Chemists' Society
Issue number4
StatePublished - Apr 1 1993


  • Caffeic acid
  • HPLC
  • electrochemical detection
  • homovanillic acid
  • hydroxytyrosol
  • olive oil
  • p-hydroxyphenylacetic acid
  • tyrosol


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