High osmolarity and L-DOPA augment release via the fusion pore in PC12 cells

Leslie A. Sombers, Nathan J. Wittenberg, Marc M. Maxson, Kelly L. Adams, Andrew G. Ewing

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

We have amperometrically measured dopamine release from rat pheochromocytoma cells (PC12 cells) in high osmolarity conditions with and without L-3,4-dihydroxyphenylalanine (L-DOPA) treatment. We observe an increase in the number of release events displaying a prespike feature or "foot" when the cells are stimulated in high osmolarity saline. We also see an increase in foot area and duration when cells are stimulated in high osmolarity saline, or high osmolarity saline subsequent to incubation with the dopamine precursor L-DOPA in isotonic saline, which serves to increase the vesicle size. The data suggest that membrane biophysics are an important component in defining the rate, duration and amount of neurotransmitter release via the fusion pore.

Original languageEnglish (US)
Pages (from-to)2471-2477
Number of pages7
JournalChemPhysChem
Volume8
Issue number17
DOIs
StatePublished - Dec 3 2007

Keywords

  • Amperometry
  • Catecholamines
  • Fusion pores
  • Hyperosmotic solutions
  • Membranes

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