High glucose induces suppression of insulin signalling and apoptosis via upregulation of endogenous IL-1β and suppressor of cytokine signalling-1 in mouse pancreatic beta cells

Panagiotis D. Venieratos, Garyfalia I. Drossopoulou, Katerina D. Kapodistria, Effie C. Tsilibary, Paraskevi V. Kitsiou

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

Chronic hyperglycemia and inflammatory cytokines disrupt and/or attenuate signal transduction pathways that promote normal β-cell survival, leading to the destruction of endocrine pancreas in type 2 diabetes. There is convincing evidence that autocrine insulin signalling exerts protective anti-apoptotic effects on beta cells. Suppressors of cytokine signalling (SOCS) were induced by several cytokines and inhibit insulin-initiated signal transduction. The aim of this study was to investigate whether high glucose can influence endogenous interleukin-1β (IL-1β) and SOCS expression thus affecting insulin signalling and survival in insulin-producing mouse pancreatic beta cells (βTC-6). Results showed that prolonged exposure of βTC-6 cells to increased glucose concentrations resulted in significant inhibition of insulin-induced tyrosine phosphorylation of the insulin receptor (IR), and insulin receptor substrate-2 (IRS-2) as well as PI3-kinase activation. These changes were accompanied by impaired activation of the anti-apoptotic signalling protein Akt and annulment of Akt-mediated suppression of the Forkhead family of transcription factors (FoxO) activation. Glucose-induced attenuation of IRS-2/Akt-mediated signalling was associated with increased IL-1β expression. Enhanced endogenous IL-1β specifically induced mRNA and protein expression of SOCS-1 in βTC-6 cells. Inhibition of SOCS-1 expression by SOCS-1-specific small interfering RNA restored IRS-2/PI3K-mediated Akt phosphorylation suppressed by high glucose. The upregulation of endogenous cytokine signalling and FoxO activation were accompanied by enhanced caspase-3 activation and increased susceptibility of cells to apoptosis. These results indicated that glucose-induced endogenous IL-1β expression increased βTC-6 cells apoptosis by inhibiting, at least in part, IRS-2/Akt-mediated signalling through SOCS-1 upregulation.

Original languageEnglish (US)
Pages (from-to)791-800
Number of pages10
JournalCellular Signalling
Volume22
Issue number5
DOIs
StatePublished - May 1 2010

Keywords

  • Apoptosis
  • IL-1β
  • Insulin signalling
  • Pancreatic beta cell glucotoxicity
  • SOCS-1
  • βTC-6 cells

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