High-density genome-wide association mapping implicates an F-box encoding gene in Medicago truncatula resistance to Aphanomyces euteiches

Maxime Bonhomme, Olivier André, Yacine Badis, Joëlle Ronfort, Concetta Burgarella, Nathalie Chantret, Jean Marie Prosperi, Roman Briskine, Joann Mudge, Frédéric Debéllé, Hélène Navier, Henri Miteul, Ahmed Hajri, Alain Baranger, Peter L Tiffin, Bernard Dumas, Marie Laure Pilet-Nayel, Nevin D Young, Christophe Jacquet

Research output: Contribution to journalArticlepeer-review

50 Scopus citations

Abstract

The use of quantitative disease resistance (QDR) is a promising strategy for promoting durable resistance to plant pathogens, but genes involved in QDR are largely unknown. To identify genetic components and accelerate improvement of QDR in legumes to the root pathogen Aphanomyces euteiches, we took advantage of both the recently generated massive genomic data for Medicago truncatula and natural variation of this model legume. A high-density (≈5.1 million single nucleotide polymorphisms (SNPs)) genome-wide association study (GWAS) was performed with both in vitro and glasshouse phenotyping data collected for 179 lines. GWAS identified several candidate genes and pinpointed two independent major loci on the top of chromosome 3 that were detected in both phenotyping methods. Candidate SNPs in the most significant locus (σA2 = 23%) were in the promoter and coding regions of an F-box protein coding gene. Subsequent qRT-PCR and bioinformatic analyses performed on 20 lines demonstrated that resistance is associated with mutations directly affecting the interaction domain of the F-box protein rather than gene expression. These results refine the position of previously identified QTL to specific candidate genes, suggest potential molecular mechanisms, and identify new loci explaining QDR against A. euteiches.

Original languageEnglish (US)
Pages (from-to)1328-1342
Number of pages15
JournalNew Phytologist
Volume201
Issue number4
DOIs
StatePublished - Mar 2014

Keywords

  • Aphanomyces euteiches
  • F-box protein
  • Genome-wide association study
  • Medicago truncatula
  • Oomycete
  • Partial resistance
  • Root

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