High density genetic maps of St. Augustinegrass and applications to comparative genomic analysis and QTL mapping for turf quality traits

Xingwang Yu, Jennifer Kimball, Susana R. Milla-Lewis

Research output: Contribution to journalArticle

Abstract

Background: St. Augustinegrass [Stenotaphrum secundatum (Walt.) Kuntze] is a warm-season, perennial turfgrass species well adapted for home lawns and commercial landscapes with economic and ecological value. However, a lack of genomic resources in St. Augustinegrass has hindered the full utilization of genetic variance for maximizing genetic gain and limited our understanding of the species' evolution. Results: In this study, we constructed the first high-density linkage map for St. Augustinegrass using a genotyping by sequencing (GBS) approach. The integrated linkage map consists of 2871 single nucleotide polymorphism (SNP) and 81 simple sequence repeat (SSR) markers, spanning 1241.7 cM, with an average distance of 0.4 cM between markers, and thus represents the densest genetic map for St. Augustinegrass to date. Comparative genomic analysis revealed inter-chromosome arrangements and independent nested chromosome fusion events that occurred after St. Augustinegrass, foxtail millet, sorghum, and rice diverged from a common ancestor. Forty-eight candidate quantitative trait loci (QTL) were detected for turf quality-related traits, including overall turf quality, leaf texture, genetic color, and turf density. Three hot spot regions were identified on linkage groups LG3 and LG8, where multi-QTL for different traits overlapped. Several leaf development related genes were contained within these identified QTL regions. Conclusions: This study developed the first high-density genetic map and identified putative QTL related to turf quality, which provide valuable genetic resources for marker-assisted selection (MAS) in St. Augustinegrass.

Original languageEnglish (US)
Article number346
JournalBMC plant biology
Volume18
Issue number1
DOIs
StatePublished - Dec 12 2018

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lawns and turf
quantitative trait loci
genomics
chromosome mapping
Stenotaphrum secundatum
chromosomes
Setaria italica
ecological value
turf grasses
leaf development
warm season
genetic variance
genetic resources
linkage groups
Sorghum (Poaceae)
economic valuation
marker-assisted selection
genotyping
single nucleotide polymorphism
genetic improvement

Keywords

  • Comparative genomic analysis
  • Linkage map
  • QTL
  • St. Augustinegrass

PubMed: MeSH publication types

  • Journal Article

Cite this

High density genetic maps of St. Augustinegrass and applications to comparative genomic analysis and QTL mapping for turf quality traits. / Yu, Xingwang; Kimball, Jennifer; Milla-Lewis, Susana R.

In: BMC plant biology, Vol. 18, No. 1, 346, 12.12.2018.

Research output: Contribution to journalArticle

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