High- and ultrahigh-field magnetic resonance imaging of naïve, injured and scarred vocal fold mucosae in rats

Ayami Ohno Kishimoto, Yo Kishimoto, David L. Young, Jinjin Zhang, Ian J. Rowland, Nathan V. Welham

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Subepithelial changes to the vocal fold mucosa, such as fibrosis, are difficult to identify using visual assessment of the tissue surface. Moreover, without suspicion of neoplasm, mucosal biopsy is not a viable clinical option, as it carries its own risk of iatrogenic injury and scar formation. Given these challenges, we assessed the ability of high- (4.7 T) and ultrahigh-field (9.4 T) magnetic resonance imaging to resolve key vocal fold subepithelial tissue structures in the rat, an important and widely used preclinical model in vocal fold biology. We conducted serial in vivo and ex vivo imaging, evaluated an array of acquisition sequences and contrast agents, and successfully resolved key anatomic features of naïve, acutely injured, and chronically scarred vocal fold mucosae on the ex vivo scans. Naïve lamina propria was hyperintense on T1-weighted imaging with gadobenate dimeglumine contrast enhancement, whereas chronic scar was characterized by reduced lamina propria T1 signal intensity and mucosal volume. Acutely injured mucosa was hypointense on T2-weighted imaging; lesion volume steadily increased, peaked at 5 days post-injury, and then decreased - consistent with the physiology of acute, followed by subacute, hemorrhage and associated changes in the magnetic state of hemoglobin and its degradation products. Intravenous administration of superparamagnetic iron oxide conferred no T2 contrast enhancement during the acute injury period. These findings confirm that magnetic resonance imaging can resolve anatomic substructures within naïve vocal fold mucosa, qualitative and quantitative features of acute injury, and the presence of chronic scar.

Original languageEnglish (US)
Pages (from-to)1397-1403
Number of pages7
JournalDMM Disease Models and Mechanisms
Volume9
Issue number11
DOIs
StatePublished - Nov 1 2016

Fingerprint

Vocal Cords
Magnetic resonance
Rats
Mucous Membrane
Magnetic Resonance Imaging
Imaging techniques
Cicatrix
Wounds and Injuries
Tissue
Biopsy
Physiology
Intravenous Administration
Contrast Media
Hemoglobins
Fibrosis
Hemorrhage
Degradation
Neoplasms

Keywords

  • Fibrosis
  • Hemorrhage
  • Larynx
  • MRI
  • Tissue repair
  • Voice
  • Wound healing

Cite this

Kishimoto, A. O., Kishimoto, Y., Young, D. L., Zhang, J., Rowland, I. J., & Welham, N. V. (2016). High- and ultrahigh-field magnetic resonance imaging of naïve, injured and scarred vocal fold mucosae in rats. DMM Disease Models and Mechanisms, 9(11), 1397-1403. https://doi.org/10.1242/dmm.026526

High- and ultrahigh-field magnetic resonance imaging of naïve, injured and scarred vocal fold mucosae in rats. / Kishimoto, Ayami Ohno; Kishimoto, Yo; Young, David L.; Zhang, Jinjin; Rowland, Ian J.; Welham, Nathan V.

In: DMM Disease Models and Mechanisms, Vol. 9, No. 11, 01.11.2016, p. 1397-1403.

Research output: Contribution to journalArticle

Kishimoto, AO, Kishimoto, Y, Young, DL, Zhang, J, Rowland, IJ & Welham, NV 2016, 'High- and ultrahigh-field magnetic resonance imaging of naïve, injured and scarred vocal fold mucosae in rats', DMM Disease Models and Mechanisms, vol. 9, no. 11, pp. 1397-1403. https://doi.org/10.1242/dmm.026526
Kishimoto, Ayami Ohno ; Kishimoto, Yo ; Young, David L. ; Zhang, Jinjin ; Rowland, Ian J. ; Welham, Nathan V. / High- and ultrahigh-field magnetic resonance imaging of naïve, injured and scarred vocal fold mucosae in rats. In: DMM Disease Models and Mechanisms. 2016 ; Vol. 9, No. 11. pp. 1397-1403.
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