Hidden IgA subclass determinants in human renal disease

T. R. Melvin; Y. Kim; M. E. Conley; D. L. Delacroix; A. F. Michael

doi:10.1038/ki.1985.65

Hidden IgA subclass determinants in human renal disease

T. R. Melvin, Y. Kim, M. E. Conley, D. L. Delacroix, A. F. Michael

Pediatric Nephrology

Research output: Contribution to journal › Article › peer-review

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Abstract

Antibodies specific for different regions of the IgA₁ molecule were used to evaluate tissue from patients with systemic lupus erythematosus (SLE), IgA nephropathy (IgAN), idiopathic membranous nephropathy (IMN), and anti-glomerular basement membrane (GBM) nephritis. One of the IgA₁ epitopes was 'masked' in subepithelial and linear BM deposits but was detected in mesangial/subendothelial deposits. This epitope was also hidden following binding of IgA₁ antinuclear antibodies (ANA) to nuclear substrates, in vitro. The presence of IgA₁ in all loci was confirmed by reactivity of two other monoclonal anti-IgA₁ antibodies.

Original language	English (US)
Pages (from-to)	685-689
Number of pages	5
Journal	Kidney international
Volume	27
Issue number	4
DOIs	https://doi.org/10.1038/ki.1985.65
State	Published - 1985

Bibliographical note

Funding Information:
This work was supported in part by grants from the National Institutes of Health (AM07087, A110704, AM22518), the Viking Children's Fund, and the American Heart Association, Minnesota Affiliate. The authors thank Ms. K. Divine, Ms. C. Blocher, and Ms. K. Pinkham for technical assistance; Mr. M. Hoff for the preparation of figures; and Ms. C. Dawis for typing the manuscript.

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title = "Hidden IgA subclass determinants in human renal disease",

abstract = "Antibodies specific for different regions of the IgA1 molecule were used to evaluate tissue from patients with systemic lupus erythematosus (SLE), IgA nephropathy (IgAN), idiopathic membranous nephropathy (IMN), and anti-glomerular basement membrane (GBM) nephritis. One of the IgA1 epitopes was 'masked' in subepithelial and linear BM deposits but was detected in mesangial/subendothelial deposits. This epitope was also hidden following binding of IgA1 antinuclear antibodies (ANA) to nuclear substrates, in vitro. The presence of IgA1 in all loci was confirmed by reactivity of two other monoclonal anti-IgA1 antibodies.",

author = "Melvin, {T. R.} and Y. Kim and Conley, {M. E.} and Delacroix, {D. L.} and Michael, {A. F.}",

note = "Funding Information: This work was supported in part by grants from the National Institutes of Health (AM07087, A110704, AM22518), the Viking Children's Fund, and the American Heart Association, Minnesota Affiliate. The authors thank Ms. K. Divine, Ms. C. Blocher, and Ms. K. Pinkham for technical assistance; Mr. M. Hoff for the preparation of figures; and Ms. C. Dawis for typing the manuscript.",

year = "1985",

doi = "10.1038/ki.1985.65",

language = "English (US)",

volume = "27",

pages = "685--689",

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T1 - Hidden IgA subclass determinants in human renal disease

AU - Melvin, T. R.

AU - Kim, Y.

AU - Conley, M. E.

AU - Delacroix, D. L.

AU - Michael, A. F.

N1 - Funding Information: This work was supported in part by grants from the National Institutes of Health (AM07087, A110704, AM22518), the Viking Children's Fund, and the American Heart Association, Minnesota Affiliate. The authors thank Ms. K. Divine, Ms. C. Blocher, and Ms. K. Pinkham for technical assistance; Mr. M. Hoff for the preparation of figures; and Ms. C. Dawis for typing the manuscript.

PY - 1985

Y1 - 1985

N2 - Antibodies specific for different regions of the IgA1 molecule were used to evaluate tissue from patients with systemic lupus erythematosus (SLE), IgA nephropathy (IgAN), idiopathic membranous nephropathy (IMN), and anti-glomerular basement membrane (GBM) nephritis. One of the IgA1 epitopes was 'masked' in subepithelial and linear BM deposits but was detected in mesangial/subendothelial deposits. This epitope was also hidden following binding of IgA1 antinuclear antibodies (ANA) to nuclear substrates, in vitro. The presence of IgA1 in all loci was confirmed by reactivity of two other monoclonal anti-IgA1 antibodies.

AB - Antibodies specific for different regions of the IgA1 molecule were used to evaluate tissue from patients with systemic lupus erythematosus (SLE), IgA nephropathy (IgAN), idiopathic membranous nephropathy (IMN), and anti-glomerular basement membrane (GBM) nephritis. One of the IgA1 epitopes was 'masked' in subepithelial and linear BM deposits but was detected in mesangial/subendothelial deposits. This epitope was also hidden following binding of IgA1 antinuclear antibodies (ANA) to nuclear substrates, in vitro. The presence of IgA1 in all loci was confirmed by reactivity of two other monoclonal anti-IgA1 antibodies.

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Hidden IgA subclass determinants in human renal disease

Abstract

Bibliographical note

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