TY - JOUR
T1 - Heterogeneity in leukemia cell populations
T2 - A clear rationale for use of combination protocols for ex vivo marrow purging
AU - Uckun, F. M.
AU - Ramakrishnan, S.
AU - Haag, D.
AU - Houston, L. L.
PY - 1985
Y1 - 1985
N2 - Immunotoxins, conjugates of monoclonal antibodies with toxins (such as ricin), a portion of the toxin (such as ricin A-chain), or a hemitoxin (such as pokeweed antiviral protein [PAP](3-6)), are currently being investigated for their value as selective marrow-purging agents in autologous bone marrow transplantation (ABMT). The efficiency of immunotoxins is likely to depend upon the phenotype of the treated leukemia cells and the extent of heterogeneity in expression of the target antigen among the leukemic cell population. In the present study, we tried to further characterize leukemic cell clones that escaped treatment with immunotoxin. Studies were performed to evaluate the ex vivo efficiency of a combination of mafosfamid, a synthetic derivative of cyclophosphamide, and a human B cell-specific immunotoxin containing PAP (B43-PAP), to eradicate these cells.
AB - Immunotoxins, conjugates of monoclonal antibodies with toxins (such as ricin), a portion of the toxin (such as ricin A-chain), or a hemitoxin (such as pokeweed antiviral protein [PAP](3-6)), are currently being investigated for their value as selective marrow-purging agents in autologous bone marrow transplantation (ABMT). The efficiency of immunotoxins is likely to depend upon the phenotype of the treated leukemia cells and the extent of heterogeneity in expression of the target antigen among the leukemic cell population. In the present study, we tried to further characterize leukemic cell clones that escaped treatment with immunotoxin. Studies were performed to evaluate the ex vivo efficiency of a combination of mafosfamid, a synthetic derivative of cyclophosphamide, and a human B cell-specific immunotoxin containing PAP (B43-PAP), to eradicate these cells.
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M3 - Article
AN - SCOPUS:0022006094
SN - 0041-1345
VL - 17
SP - 462
EP - 464
JO - Transplantation proceedings
JF - Transplantation proceedings
IS - 1 I
ER -