Heterogeneity in human cardiac troponin I standards

David M. Bunk, Joseph J. Dalluge, Michael J. Welch

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

The LC-MS analysis of recombinant cardiac troponin I (cTnI) and cTnI extracted from human hearts showed a high degree of structural heterogeneity among all samples. The examined recombinant cTnI samples indicated posttranslational modifications, presumably due to their purification (i.e., 2-mercaptoethanol adducts and carbamylation) and related to their expression (i.e., an N-terminal expression tag). The extracted cTnI samples, while having a higher degree of structural heterogeneity, showed less structural variance between samples than the recombinant proteins. The LC-MS analysis of the extracted cTnI samples provided evidence of posttranslational modification by phosphorylation, acetylation, proteolytic cleavage, and intrachain disulfide bond formation.

Original languageEnglish (US)
Pages (from-to)191-200
Number of pages10
JournalAnalytical Biochemistry
Volume284
Issue number2
DOIs
StatePublished - Sep 10 2000

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