Heterogeneity in human cardiac troponin I standards

David M. Bunk, Joseph J. Dalluge, Michael J. Welch

Research output: Contribution to journalArticlepeer-review

27 Scopus citations


The LC-MS analysis of recombinant cardiac troponin I (cTnI) and cTnI extracted from human hearts showed a high degree of structural heterogeneity among all samples. The examined recombinant cTnI samples indicated posttranslational modifications, presumably due to their purification (i.e., 2-mercaptoethanol adducts and carbamylation) and related to their expression (i.e., an N-terminal expression tag). The extracted cTnI samples, while having a higher degree of structural heterogeneity, showed less structural variance between samples than the recombinant proteins. The LC-MS analysis of the extracted cTnI samples provided evidence of posttranslational modification by phosphorylation, acetylation, proteolytic cleavage, and intrachain disulfide bond formation.

Original languageEnglish (US)
Pages (from-to)191-200
Number of pages10
JournalAnalytical Biochemistry
Issue number2
StatePublished - Sep 10 2000


Dive into the research topics of 'Heterogeneity in human cardiac troponin I standards'. Together they form a unique fingerprint.

Cite this