Hepatic mixed-function oxidase activity in rainbow trout exposed to several polycyclic aromatic compounds

Ellen Heath Gerhart, Robert M Carlson

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75 Scopus citations


The effects of polycyclic aromatic hydrocarbons (PAHs) on microsomal liver enzymes were examined in rainbow trout. Various PAHs (naphthalene through benzo(a)pyrene [B(aP]) were injected ip to screen for mixed-function oxidase (MFO) activity. Chrysene, B(a)P, and Aroclor 1254 caused MFO induction. When fish were also exposed to solubilized pyrene, fluoranthene, and B(a)P in water, bioaccumulation of B(a)P resulted in MFO induction, whereas bioaccumulation of pyrene and fluoranthene did not. Based on water and injection exposure to B(a)P, it was predicted that tissue concentrations in excess of 300 μg/kg B(a)P would be accompanied by MFO induction in rainbow trout.

Original languageEnglish (US)
Pages (from-to)284-295
Number of pages12
JournalEnvironmental Research
Issue number2
StatePublished - Oct 1978

Bibliographical note

Funding Information:
Space for the bioassays and the unlimited use of equipment was generously provided by the Environmental Research Laboratory-Duluth (ERL-D). We greatly appreciate the assistance of the ERL-D staff, particularly Drs. G. M. Christensen and J. M. McKim for helpful discussions and Ms. B. J. Halligan for preparing the figures. We also wish to thank Ms. Patricia A. Kosian for providing excellent technical assistance and to express our appreciation to Dr. A. R. Oyler for consultation on the analytical methodology and to Dr. Ronald Caple and Mr. Dale Swanson for the sample of 1,2,4-trimethylnaphthalene. This research was supported by EPA Grant R803952-03-I.


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