Hepatic and cardiac carnitine palmitoyltransferase activity. Effects of adriamycin and galactosamine

Linda J. Brady; Paul S. Brady

doi:10.1016/0006-2952(87)90320-0

Hepatic and cardiac carnitine palmitoyltransferase activity. Effects of adriamycin and galactosamine

Linda J. Brady
, Paul S. Brady

Food Science and Nutrition

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

Carnitine palmitoyltransferase (CPT) activity is located on both the outer and inner sides of the mitochondrial inner membrane and is influenced by the surrounding lipids of the inner mitochondrial membrane. Both adriamycin and galactosamine interact with mitochondrial lipids as a part of their mechanism of toxicity, and thus these agents might be expected to affect CPT activity. Addition of adriamycin to both intact rat liver and heart mitochondria (CPT-A, outer CPT) and inverted submitochondrial vesicles (CPT-B, inner CPT) depressed CPT in the forward direction of reaction (palmitoyl-l-carnitine formation), but the CPT-B activity was more sensitive to the inhibitor. Adriamycin depressed the CPT-A reverse reaction (palmitoyl-CoA formation) to 40% of control, but it had no effect on the CPT-B reverse reaction. In vivo galactosamine administration depressed CPT-A and CPT-B 20-30% and did not affect subsequent action of in vitro adriamycin. Addition of cardiolipin (0.25 to 1.0 mg/ assay) increased activity of the CPT-A forward reaction of both control and galactosamine-treated rats, but it did not affect CPT-B activity. The results suggest that CPT-A and CPT-B may be influenced differently by pertubants that affect lipids of the membrane.

Original language	English (US)
Pages (from-to)	3419-3423
Number of pages	5
Journal	Biochemical Pharmacology
Volume	36
Issue number	20
DOIs	https://doi.org/10.1016/0006-2952(87)90320-0
State	Published - Oct 15 1987

Bibliographical note

Funding Information:
*This work was supported by the American Heart Association, Washington Affiliate, and the Agriculture and Home Economics Research Center of Washington State University. Scientific Paper No. 7671. TPresent address: Department of Food Science and Nutrition, University of Minnesota, 1334 Eckles Ave., St. Paul, MN 55108. *Author to whom correspondence should be addressed. §CFT-A refers to the carnitine palmitoyltransferase activity occurring on the outer side of the inner mitochondrial membrane. This activity has also been referred to as CPT-I, CPT, or outer CPT. CPT-B refers to the carnitine palmitoyltransferase activity occurring on the inner side of the mitochondrial inner membrane. This activity has also been referred to as CPT-II, CPTi or inner CPT.

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@article{55a85b9b6d8446b39fd810898faa231e,

title = "Hepatic and cardiac carnitine palmitoyltransferase activity. Effects of adriamycin and galactosamine",

abstract = "Carnitine palmitoyltransferase (CPT) activity is located on both the outer and inner sides of the mitochondrial inner membrane and is influenced by the surrounding lipids of the inner mitochondrial membrane. Both adriamycin and galactosamine interact with mitochondrial lipids as a part of their mechanism of toxicity, and thus these agents might be expected to affect CPT activity. Addition of adriamycin to both intact rat liver and heart mitochondria (CPT-A, outer CPT) and inverted submitochondrial vesicles (CPT-B, inner CPT) depressed CPT in the forward direction of reaction (palmitoyl-l-carnitine formation), but the CPT-B activity was more sensitive to the inhibitor. Adriamycin depressed the CPT-A reverse reaction (palmitoyl-CoA formation) to 40\% of control, but it had no effect on the CPT-B reverse reaction. In vivo galactosamine administration depressed CPT-A and CPT-B 20-30\% and did not affect subsequent action of in vitro adriamycin. Addition of cardiolipin (0.25 to 1.0 mg/ assay) increased activity of the CPT-A forward reaction of both control and galactosamine-treated rats, but it did not affect CPT-B activity. The results suggest that CPT-A and CPT-B may be influenced differently by pertubants that affect lipids of the membrane.",

author = "Brady, \{Linda J.\} and Brady, \{Paul S.\}",

note = "Funding Information: *This work was supported by the American Heart Association, Washington Affiliate, and the Agriculture and Home Economics Research Center of Washington State University. Scientific Paper No. 7671. TPresent address: Department of Food Science and Nutrition, University of Minnesota, 1334 Eckles Ave., St. Paul, MN 55108. *Author to whom correspondence should be addressed. §CFT-A refers to the carnitine palmitoyltransferase activity occurring on the outer side of the inner mitochondrial membrane. This activity has also been referred to as CPT-I, CPT, or outer CPT. CPT-B refers to the carnitine palmitoyltransferase activity occurring on the inner side of the mitochondrial inner membrane. This activity has also been referred to as CPT-II, CPTi or inner CPT.",

year = "1987",

month = oct,

day = "15",

doi = "10.1016/0006-2952(87)90320-0",

language = "English (US)",

volume = "36",

pages = "3419--3423",

journal = "Biochemical Pharmacology",

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T1 - Hepatic and cardiac carnitine palmitoyltransferase activity. Effects of adriamycin and galactosamine

AU - Brady, Linda J.

AU - Brady, Paul S.

N1 - Funding Information: *This work was supported by the American Heart Association, Washington Affiliate, and the Agriculture and Home Economics Research Center of Washington State University. Scientific Paper No. 7671. TPresent address: Department of Food Science and Nutrition, University of Minnesota, 1334 Eckles Ave., St. Paul, MN 55108. *Author to whom correspondence should be addressed. §CFT-A refers to the carnitine palmitoyltransferase activity occurring on the outer side of the inner mitochondrial membrane. This activity has also been referred to as CPT-I, CPT, or outer CPT. CPT-B refers to the carnitine palmitoyltransferase activity occurring on the inner side of the mitochondrial inner membrane. This activity has also been referred to as CPT-II, CPTi or inner CPT.

PY - 1987/10/15

Y1 - 1987/10/15

N2 - Carnitine palmitoyltransferase (CPT) activity is located on both the outer and inner sides of the mitochondrial inner membrane and is influenced by the surrounding lipids of the inner mitochondrial membrane. Both adriamycin and galactosamine interact with mitochondrial lipids as a part of their mechanism of toxicity, and thus these agents might be expected to affect CPT activity. Addition of adriamycin to both intact rat liver and heart mitochondria (CPT-A, outer CPT) and inverted submitochondrial vesicles (CPT-B, inner CPT) depressed CPT in the forward direction of reaction (palmitoyl-l-carnitine formation), but the CPT-B activity was more sensitive to the inhibitor. Adriamycin depressed the CPT-A reverse reaction (palmitoyl-CoA formation) to 40% of control, but it had no effect on the CPT-B reverse reaction. In vivo galactosamine administration depressed CPT-A and CPT-B 20-30% and did not affect subsequent action of in vitro adriamycin. Addition of cardiolipin (0.25 to 1.0 mg/ assay) increased activity of the CPT-A forward reaction of both control and galactosamine-treated rats, but it did not affect CPT-B activity. The results suggest that CPT-A and CPT-B may be influenced differently by pertubants that affect lipids of the membrane.

AB - Carnitine palmitoyltransferase (CPT) activity is located on both the outer and inner sides of the mitochondrial inner membrane and is influenced by the surrounding lipids of the inner mitochondrial membrane. Both adriamycin and galactosamine interact with mitochondrial lipids as a part of their mechanism of toxicity, and thus these agents might be expected to affect CPT activity. Addition of adriamycin to both intact rat liver and heart mitochondria (CPT-A, outer CPT) and inverted submitochondrial vesicles (CPT-B, inner CPT) depressed CPT in the forward direction of reaction (palmitoyl-l-carnitine formation), but the CPT-B activity was more sensitive to the inhibitor. Adriamycin depressed the CPT-A reverse reaction (palmitoyl-CoA formation) to 40% of control, but it had no effect on the CPT-B reverse reaction. In vivo galactosamine administration depressed CPT-A and CPT-B 20-30% and did not affect subsequent action of in vitro adriamycin. Addition of cardiolipin (0.25 to 1.0 mg/ assay) increased activity of the CPT-A forward reaction of both control and galactosamine-treated rats, but it did not affect CPT-B activity. The results suggest that CPT-A and CPT-B may be influenced differently by pertubants that affect lipids of the membrane.

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DO - 10.1016/0006-2952(87)90320-0

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SN - 0006-2952

VL - 36

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JO - Biochemical Pharmacology

JF - Biochemical Pharmacology

IS - 20

ER -

Hepatic and cardiac carnitine palmitoyltransferase activity. Effects of adriamycin and galactosamine

Abstract

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