Hemoglobin oxidation-dependent reactions promote interactions with band 3 and oxidative changes in sickle cell-derived microparticles

Sirsendu Jana, Michael Brad Strader, Fantao Meng, Wayne Hicks, Tigist Kassa, Ivan Tarandovskiy, Silvia De Paoli, Jan Simak, Michael R. Heaven, John D Belcher, Gregory M Vercellotti, Abdu I. Alayash

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The contribution of intracellular hemoglobin (Hb) oxidation to RBC-derived microparticle (MP) formation is poorly defined in sickle cell disease (SCD). Here we report that sickle Hb (HbS) oxidation, coupled with changes in cytosolic antioxidative proteins, is associated with membrane alterations and MP formation in homozygous Townes-sickle cell (Townes-SS) mice. Photometric and proteomic analyses confirmed the presence of high levels of Hb oxidation intermediates (ferric/ferryl) and consequent β-globin posttranslational modifications, including the irreversible oxidation of βCys93 and the ubiquitination of βLys96 and βLys145. This is the first report to our knowledge to link the UPS (via ubiquitinated Hb and other proteins) to oxidative stress. Ferryl Hb also induced complex formation with band 3 and RBC membrane proteins. Incubation of Townes-SS MPs with human endothelial cells caused greater loss of monolayer integrity, apoptotic activation, heme oxygenase-1 induction, and concomitant bioenergetic imbalance compared with control Townes-AA MPs. MPs obtained from Townes-SS mice treated with hydroxyurea produced fewer posttranslational Hb modifications. In vitro, hydroxyurea reduced the levels of ferryl Hb and shielded its target residue, βCys93, by a process of S-nitrosylation. These mechanistic analyses suggest potential antioxidative therapeutic modalities that may interrupt MP heme-mediated pathophysiology in SCD patients.

Original languageEnglish (US)
JournalJCI insight
Volume3
Issue number21
DOIs
StatePublished - Nov 2 2018

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Cell-Derived Microparticles
Hemoglobins
Hydroxyurea
Sickle Cell Anemia
Post Translational Protein Processing
Sickle Hemoglobin
Heme Oxygenase-1
Globins
Ubiquitination
Heme
Proteomics
Energy Metabolism
Membrane Proteins
Proteins
Oxidative Stress
Endothelial Cells
Membranes

Keywords

  • Cardiovascular disease
  • Hematology
  • Proteomics
  • Ubiquitin-proteosome system
  • Vascular Biology

PubMed: MeSH publication types

  • Journal Article

Cite this

Hemoglobin oxidation-dependent reactions promote interactions with band 3 and oxidative changes in sickle cell-derived microparticles. / Jana, Sirsendu; Strader, Michael Brad; Meng, Fantao; Hicks, Wayne; Kassa, Tigist; Tarandovskiy, Ivan; De Paoli, Silvia; Simak, Jan; Heaven, Michael R.; Belcher, John D; Vercellotti, Gregory M; Alayash, Abdu I.

In: JCI insight, Vol. 3, No. 21, 02.11.2018.

Research output: Contribution to journalArticle

Jana, S, Strader, MB, Meng, F, Hicks, W, Kassa, T, Tarandovskiy, I, De Paoli, S, Simak, J, Heaven, MR, Belcher, JD, Vercellotti, GM & Alayash, AI 2018, 'Hemoglobin oxidation-dependent reactions promote interactions with band 3 and oxidative changes in sickle cell-derived microparticles', JCI insight, vol. 3, no. 21. https://doi.org/10.1172/jci.insight.120451
Jana, Sirsendu ; Strader, Michael Brad ; Meng, Fantao ; Hicks, Wayne ; Kassa, Tigist ; Tarandovskiy, Ivan ; De Paoli, Silvia ; Simak, Jan ; Heaven, Michael R. ; Belcher, John D ; Vercellotti, Gregory M ; Alayash, Abdu I. / Hemoglobin oxidation-dependent reactions promote interactions with band 3 and oxidative changes in sickle cell-derived microparticles. In: JCI insight. 2018 ; Vol. 3, No. 21.
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