TY - JOUR
T1 - Hematopoietic reconstitution by multipotent adult progenitor cells
T2 - Precursors to long-term hematopoietic stem cells
AU - Serafini, Marta
AU - Dylla, Scott J.
AU - Oki, Masayuki
AU - Heremans, Yves
AU - Tolar, Jakub
AU - Jiang, Yuehua
AU - Buckley, Shannon M.
AU - Pelacho, Beatriz
AU - Burns, Terry C.
AU - Frommer, Sarah
AU - Rossi, Derrick J.
AU - Bryder, David
AU - Panoskaltsis-Mortari, Angela
AU - O'Shaughnessy, Matthew J.
AU - Nelson-Holte, Molly
AU - Fine, Gabriel C.
AU - Weissman, Irving L.
AU - Blazar, Bruce R.
AU - Verfaillie, Catherine M.
PY - 2007/1/22
Y1 - 2007/1/22
N2 - For decades, in vitro expansion of transplantable hematopoietic stem cells (HSCs) has been an elusive goal. Here, we demonstrate that multipotent adult progenitor cells (MAPCs), isolated from green fluorescent protein (GFP)-transgenic mice and expanded in vitro for >40-80 population doublings, are capable of multilineage hematopoietic engraftment of immunodeficient mice. Among MAPC-derived GFP+CD45.2+ cells in the bone marrow of engrafted mice, HSCs were present that could radioprotect and reconstitute multilineage hematopoiesis in secondary and tertiary recipients, as well as myeloid and lymphoid hematopoietic progenitor subsets and functional GFP + MAPC-derived lymphocytes that were functional. Although hematopoietic contribution by MAPCs was comparable to control KTLS HSCs, approximately 103-fold more MAPCs were required for efficient engraftment. Because GFP+ host-derived CD45.1+ cells were not observed, fusion is not likely to account for the generation of HSCs by MAPCs. JEM
AB - For decades, in vitro expansion of transplantable hematopoietic stem cells (HSCs) has been an elusive goal. Here, we demonstrate that multipotent adult progenitor cells (MAPCs), isolated from green fluorescent protein (GFP)-transgenic mice and expanded in vitro for >40-80 population doublings, are capable of multilineage hematopoietic engraftment of immunodeficient mice. Among MAPC-derived GFP+CD45.2+ cells in the bone marrow of engrafted mice, HSCs were present that could radioprotect and reconstitute multilineage hematopoiesis in secondary and tertiary recipients, as well as myeloid and lymphoid hematopoietic progenitor subsets and functional GFP + MAPC-derived lymphocytes that were functional. Although hematopoietic contribution by MAPCs was comparable to control KTLS HSCs, approximately 103-fold more MAPCs were required for efficient engraftment. Because GFP+ host-derived CD45.1+ cells were not observed, fusion is not likely to account for the generation of HSCs by MAPCs. JEM
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U2 - 10.1084/jem.20061115
DO - 10.1084/jem.20061115
M3 - Article
C2 - 17227908
AN - SCOPUS:33846437812
SN - 0022-1007
VL - 204
SP - 129
EP - 139
JO - Journal of Experimental Medicine
JF - Journal of Experimental Medicine
IS - 1
ER -