Hematopoietic development of human embryonic stem cells in culture

Xinghui Tian, Dan S. Kaufman

Research output: Chapter in Book/Report/Conference proceedingChapter

15 Scopus citations

Abstract

The successful isolation and characterization of human embryonic stem cells (hESCs) provides a powerful tool to study the cellular and genetic mechanisms that mediate cell-fate decisions toward distinct developmental lineages. hESC-derived cells may also be suitable for novel cellular therapies. Significant progress in hematopoietic development of hESCs has demonstrated production of many types of blood cells from hESCs including myeloid, erythroid and lymphoid lineage cells, and possibly hematopoietic stem cells. Current established approaches to generate specific hematopoietic lineages are based on the initial pre-differentiation of hESCs into a heterogeneous mixture of cell populations. In this chapter, we describe two methods that have been successfully used in our laboratory: (1) co-culture with stromal cells derived from, hematopoietic microenvironments and (2) embryoid body (EB) formation. Subsequent to this early differentiation step, distinct progenitor cell populations can be derived, sorted, and utilized for further lineage-specific developmental studies.

Original languageEnglish (US)
Title of host publicationHematopoietic Stem Cell Protocols
Pages119-133
Number of pages15
StatePublished - Aug 31 2007

Publication series

NameMethods in Molecular Biology
Volume430
ISSN (Print)1064-3745

Keywords

  • Differentiation
  • Embryoid body
  • Hematopoiesis
  • Hematopoietic precursor
  • Stromal cell
  • hESCs

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