Hematein inhibits tumor necrotic factor-α-induced vascular cell adhesion molecule-1 and NF-κB-dependent gene expression in human vascular endothelial cells

Jung Joo Hong, Tae Sook Jeong, Jae Hoon Choi, Jae Hak Park, Kun Young Lee, Yun Jeong Seo, Sei Ryang Oh, Goo Taeg Oh

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Monocyte adhesion to the endothelium via adhesion molecules is one of the earliest events in atherogenesis. It has been suggested that vascular cell adhesion molecule-1 (VCAM-1) plays a very important role in the recruitment of monocytes in atherosclerosis. The aim of our study was to evaluate whether hematein can influence the expression of VCAM-1 and the transcription of nuclear factor-κB (NF-κB)-dependent genes. Immunohistochemistry revealed that mouse aortic artery endothelial cells express VCAM-1 after feeding a high cholesterol diet for 8 weeks. Hematein dose dependently suppressed TNF-α-induced VCAM-1 in both surface (30.8%) and soluble protein (65%) production in HUVECs. The transcription level of VCAM-1 was measured by Northern blot analysis, and decreased VCAM-1 protein expression was associated with a reduction of VCAM-1 mRNA expression. Transient transfection study of NF-κB promoter construct and electrophoretic mobility shift assay suggested that hematein inhibited both NF-κB-dependent gene expression and NF-κB activation induced by TNF-α. Our results suggest that the down-regulation of VCAM-1 expression by hematein may in part be due to the inhibition of NF-κB-dependent gene expression.

Original languageEnglish (US)
Pages (from-to)1127-1133
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume281
Issue number5
DOIs
StatePublished - 2001

Bibliographical note

Funding Information:
We extend our thanks to Dr. Minho Shong and Hyo Kyun Chung (Chungnam National University) for the helpful comments, and to Ae-ran Moon for the support during the transfection assay. This work was financially supported by the Molecular Medicine Research Group Program (99-J03-01-01-A-05) from MOST and by HMP-98-D-4-0044 from the Ministry of Health and Welfare of Korea.

Keywords

  • Endothelial cells
  • Hematein
  • Vascular cell adhesion molecule

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