Monocyte adhesion to the endothelium via adhesion molecules is one of the earliest events in atherogenesis. It has been suggested that vascular cell adhesion molecule-1 (VCAM-1) plays a very important role in the recruitment of monocytes in atherosclerosis. The aim of our study was to evaluate whether hematein can influence the expression of VCAM-1 and the transcription of nuclear factor-κB (NF-κB)-dependent genes. Immunohistochemistry revealed that mouse aortic artery endothelial cells express VCAM-1 after feeding a high cholesterol diet for 8 weeks. Hematein dose dependently suppressed TNF-α-induced VCAM-1 in both surface (30.8%) and soluble protein (65%) production in HUVECs. The transcription level of VCAM-1 was measured by Northern blot analysis, and decreased VCAM-1 protein expression was associated with a reduction of VCAM-1 mRNA expression. Transient transfection study of NF-κB promoter construct and electrophoretic mobility shift assay suggested that hematein inhibited both NF-κB-dependent gene expression and NF-κB activation induced by TNF-α. Our results suggest that the down-regulation of VCAM-1 expression by hematein may in part be due to the inhibition of NF-κB-dependent gene expression.
|Original language||English (US)|
|Number of pages||7|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - 2001|
Bibliographical noteFunding Information:
We extend our thanks to Dr. Minho Shong and Hyo Kyun Chung (Chungnam National University) for the helpful comments, and to Ae-ran Moon for the support during the transfection assay. This work was financially supported by the Molecular Medicine Research Group Program (99-J03-01-01-A-05) from MOST and by HMP-98-D-4-0044 from the Ministry of Health and Welfare of Korea.
- Endothelial cells
- Vascular cell adhesion molecule