Heat shock gene expression in germinating ascospores of Neurospora tetrasperma

N. Plesofsky-Vig; A. Paulson; E. P. Hill; L. Glaser; R. Brambl

doi:10.1016/0378-1097(92)90614-T

Heat shock gene expression in germinating ascospores of Neurospora tetrasperma

N. Plesofsky-Vig, A. Paulson, E. P. Hill, L. Glaser, R. Brambl

Plant and Microbial Biology

Research output: Contribution to journal › Article › peer-review

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Abstract

The activated ascospores of Neurospora tetrasperma were inactive in protein synthesis and did not accumulate transcripts for a constitutive protein until after 90 min of incubation. These spores were blocked even longer in the expression of a gene encoding a heat shock protein, hsp30, which could not be induced until after 300 min of spore germination. Early in germination the ascospores were highly susceptible to damage from moderately high temperatures. At the same time that spores became capable of expressing the hsp30 gene, there was a loss of cytosine methylation from the gene.

Original language	English (US)
Pages (from-to)	117-122
Number of pages	6
Journal	FEMS Microbiology Letters
Volume	90
Issue number	2
DOIs	https://doi.org/10.1016/0378-1097(92)90614-T
State	Published - Jan 1 1992

Bibliographical note

Funding Information:
This research was supported by grants from the National Institute of General Medical Sciences (GM-19398), the United States Department of Agriculture Competitive Research Grants Office (88-37264-4045). and the Bush Foundation.

Keywords

Ascospore
Cytosine methylation
Gene expression
Heat shock protein
Neurospora tetrasperma
Spore germination

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10.1016/0378-1097(92)90614-T

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title = "Heat shock gene expression in germinating ascospores of Neurospora tetrasperma",

abstract = "The activated ascospores of Neurospora tetrasperma were inactive in protein synthesis and did not accumulate transcripts for a constitutive protein until after 90 min of incubation. These spores were blocked even longer in the expression of a gene encoding a heat shock protein, hsp30, which could not be induced until after 300 min of spore germination. Early in germination the ascospores were highly susceptible to damage from moderately high temperatures. At the same time that spores became capable of expressing the hsp30 gene, there was a loss of cytosine methylation from the gene.",

keywords = "Ascospore, Cytosine methylation, Gene expression, Heat shock protein, Neurospora tetrasperma, Spore germination",

author = "N. Plesofsky-Vig and A. Paulson and Hill, {E. P.} and L. Glaser and R. Brambl",

note = "Funding Information: This research was supported by grants from the National Institute of General Medical Sciences (GM-19398), the United States Department of Agriculture Competitive Research Grants Office (88-37264-4045). and the Bush Foundation.",

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doi = "10.1016/0378-1097(92)90614-T",

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T1 - Heat shock gene expression in germinating ascospores of Neurospora tetrasperma

AU - Plesofsky-Vig, N.

AU - Paulson, A.

AU - Hill, E. P.

AU - Glaser, L.

AU - Brambl, R.

N1 - Funding Information: This research was supported by grants from the National Institute of General Medical Sciences (GM-19398), the United States Department of Agriculture Competitive Research Grants Office (88-37264-4045). and the Bush Foundation.

PY - 1992/1/1

Y1 - 1992/1/1

N2 - The activated ascospores of Neurospora tetrasperma were inactive in protein synthesis and did not accumulate transcripts for a constitutive protein until after 90 min of incubation. These spores were blocked even longer in the expression of a gene encoding a heat shock protein, hsp30, which could not be induced until after 300 min of spore germination. Early in germination the ascospores were highly susceptible to damage from moderately high temperatures. At the same time that spores became capable of expressing the hsp30 gene, there was a loss of cytosine methylation from the gene.

AB - The activated ascospores of Neurospora tetrasperma were inactive in protein synthesis and did not accumulate transcripts for a constitutive protein until after 90 min of incubation. These spores were blocked even longer in the expression of a gene encoding a heat shock protein, hsp30, which could not be induced until after 300 min of spore germination. Early in germination the ascospores were highly susceptible to damage from moderately high temperatures. At the same time that spores became capable of expressing the hsp30 gene, there was a loss of cytosine methylation from the gene.

KW - Ascospore

KW - Cytosine methylation

KW - Gene expression

KW - Heat shock protein

KW - Neurospora tetrasperma

KW - Spore germination

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Heat shock gene expression in germinating ascospores of Neurospora tetrasperma

Abstract

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