Harvesting cells under anchorage-independent cell transformation conditions for biochemical analyses.

Zigang Dong, Joan L. Cmarik

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

Most molecular biology and biochemical analyses use cultured cells grown in anchorage-dependent monolayer conditions. The standard oncogenic transformation assay for cell lines is usually performed in soft agar rather than in monolayers because of the higher transformation efficiency of cells in soft agar. However, cells suspended in soft agar cannot be readily recovered for studying inducible biochemical and molecular events. We developed an over-agar assay that enables us to study tumor promoter-induced cell transformation and the associated biochemical or molecular events under anchorage-independent conditions.

Original languageEnglish (US)
Pages (from-to)PL7
JournalScience's STKE : signal transduction knowledge environment
Volume2002
Issue number130
DOIs
StatePublished - Apr 30 2002

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