Harnessing the power of cruciferous vegetables

Developing a biomarker for brassica vegetable consumption using urinary 3,3'-diindolylmethane

Naomi Fujioka, Benjamin W. Ransom, Steven G Carmella, Pramod Upadhyaya, Bruce R. Lindgren, Astia Roper-Batker, Dorothy K Hatsukami, Vincent A Fritz, Charlie Rohwer, Stephen S Hecht

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12 Citations (Scopus)

Abstract

Glucobrassicin in Brassica vegetables gives rise to indole-3- carbinol (I3C), a compound with potent anticancer effects in preclinical models. We previously showed that the urinary metabolite 3,3'-diindolylmethane (DIM) could discriminate between volunteers fed high and low doses of Brassica vegetables. However, the quantitative relationship between glucobrassicin exposure and urinary DIM level is unclear. We conducted a clinical trial to examine the hypotheses that a range of glucobrassicin exposure from Brassica vegetables is reflected in urinary DIM and that this effect plateaus. Forty-five subjects consumed vegetables, a mixture of brussels sprouts and/or cabbage, at one of seven discrete dose levels of glucobrassicin ranging from 25 to 500 μmol, once daily for 2 consecutive days. All urine was collected for 24 hours after each vegetable-eating session. Urinary DIMwas measured using our published liquid chromatography-electrospray ionizationtandem mass spectrometry-selected reaction monitoring (LC/ ESI-MS/MS-SRM) method. Urinary DIM excretion increased predictably with increasing glucobrassicin dose and plateaued between 200 and 300 μmol of glucobrassicin. The association between glucobrassicin dose and urinary DIM was strong and positive (R2 = 0.68). The majority of DIM was excreted in the first 12 hours after vegetable consumption. We conclude that urinary DIM is a reliable biomarker of glucobrassicin exposure and I3C uptake and that feeding glucobrassicin beyond 200 mmol did not consistently lead to more urinary DIM, suggesting a plateau in potential chemopreventive benefit.

Original languageEnglish (US)
Pages (from-to)788-793
Number of pages6
JournalCancer Prevention Research
Volume9
Issue number10
DOIs
StatePublished - Oct 1 2016

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Brassica
Vegetables
Biomarkers
3,3'-diindolylmethane
glucobrassicin
diindolylmethane
Liquid Chromatography
Volunteers
Mass Spectrometry
Eating
Clinical Trials
Urine

Cite this

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title = "Harnessing the power of cruciferous vegetables: Developing a biomarker for brassica vegetable consumption using urinary 3,3'-diindolylmethane",
abstract = "Glucobrassicin in Brassica vegetables gives rise to indole-3- carbinol (I3C), a compound with potent anticancer effects in preclinical models. We previously showed that the urinary metabolite 3,3'-diindolylmethane (DIM) could discriminate between volunteers fed high and low doses of Brassica vegetables. However, the quantitative relationship between glucobrassicin exposure and urinary DIM level is unclear. We conducted a clinical trial to examine the hypotheses that a range of glucobrassicin exposure from Brassica vegetables is reflected in urinary DIM and that this effect plateaus. Forty-five subjects consumed vegetables, a mixture of brussels sprouts and/or cabbage, at one of seven discrete dose levels of glucobrassicin ranging from 25 to 500 μmol, once daily for 2 consecutive days. All urine was collected for 24 hours after each vegetable-eating session. Urinary DIMwas measured using our published liquid chromatography-electrospray ionizationtandem mass spectrometry-selected reaction monitoring (LC/ ESI-MS/MS-SRM) method. Urinary DIM excretion increased predictably with increasing glucobrassicin dose and plateaued between 200 and 300 μmol of glucobrassicin. The association between glucobrassicin dose and urinary DIM was strong and positive (R2 = 0.68). The majority of DIM was excreted in the first 12 hours after vegetable consumption. We conclude that urinary DIM is a reliable biomarker of glucobrassicin exposure and I3C uptake and that feeding glucobrassicin beyond 200 mmol did not consistently lead to more urinary DIM, suggesting a plateau in potential chemopreventive benefit.",
author = "Naomi Fujioka and Ransom, {Benjamin W.} and Carmella, {Steven G} and Pramod Upadhyaya and Lindgren, {Bruce R.} and Astia Roper-Batker and Hatsukami, {Dorothy K} and Fritz, {Vincent A} and Charlie Rohwer and Hecht, {Stephen S}",
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T1 - Harnessing the power of cruciferous vegetables

T2 - Developing a biomarker for brassica vegetable consumption using urinary 3,3'-diindolylmethane

AU - Fujioka, Naomi

AU - Ransom, Benjamin W.

AU - Carmella, Steven G

AU - Upadhyaya, Pramod

AU - Lindgren, Bruce R.

AU - Roper-Batker, Astia

AU - Hatsukami, Dorothy K

AU - Fritz, Vincent A

AU - Rohwer, Charlie

AU - Hecht, Stephen S

PY - 2016/10/1

Y1 - 2016/10/1

N2 - Glucobrassicin in Brassica vegetables gives rise to indole-3- carbinol (I3C), a compound with potent anticancer effects in preclinical models. We previously showed that the urinary metabolite 3,3'-diindolylmethane (DIM) could discriminate between volunteers fed high and low doses of Brassica vegetables. However, the quantitative relationship between glucobrassicin exposure and urinary DIM level is unclear. We conducted a clinical trial to examine the hypotheses that a range of glucobrassicin exposure from Brassica vegetables is reflected in urinary DIM and that this effect plateaus. Forty-five subjects consumed vegetables, a mixture of brussels sprouts and/or cabbage, at one of seven discrete dose levels of glucobrassicin ranging from 25 to 500 μmol, once daily for 2 consecutive days. All urine was collected for 24 hours after each vegetable-eating session. Urinary DIMwas measured using our published liquid chromatography-electrospray ionizationtandem mass spectrometry-selected reaction monitoring (LC/ ESI-MS/MS-SRM) method. Urinary DIM excretion increased predictably with increasing glucobrassicin dose and plateaued between 200 and 300 μmol of glucobrassicin. The association between glucobrassicin dose and urinary DIM was strong and positive (R2 = 0.68). The majority of DIM was excreted in the first 12 hours after vegetable consumption. We conclude that urinary DIM is a reliable biomarker of glucobrassicin exposure and I3C uptake and that feeding glucobrassicin beyond 200 mmol did not consistently lead to more urinary DIM, suggesting a plateau in potential chemopreventive benefit.

AB - Glucobrassicin in Brassica vegetables gives rise to indole-3- carbinol (I3C), a compound with potent anticancer effects in preclinical models. We previously showed that the urinary metabolite 3,3'-diindolylmethane (DIM) could discriminate between volunteers fed high and low doses of Brassica vegetables. However, the quantitative relationship between glucobrassicin exposure and urinary DIM level is unclear. We conducted a clinical trial to examine the hypotheses that a range of glucobrassicin exposure from Brassica vegetables is reflected in urinary DIM and that this effect plateaus. Forty-five subjects consumed vegetables, a mixture of brussels sprouts and/or cabbage, at one of seven discrete dose levels of glucobrassicin ranging from 25 to 500 μmol, once daily for 2 consecutive days. All urine was collected for 24 hours after each vegetable-eating session. Urinary DIMwas measured using our published liquid chromatography-electrospray ionizationtandem mass spectrometry-selected reaction monitoring (LC/ ESI-MS/MS-SRM) method. Urinary DIM excretion increased predictably with increasing glucobrassicin dose and plateaued between 200 and 300 μmol of glucobrassicin. The association between glucobrassicin dose and urinary DIM was strong and positive (R2 = 0.68). The majority of DIM was excreted in the first 12 hours after vegetable consumption. We conclude that urinary DIM is a reliable biomarker of glucobrassicin exposure and I3C uptake and that feeding glucobrassicin beyond 200 mmol did not consistently lead to more urinary DIM, suggesting a plateau in potential chemopreventive benefit.

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JO - Cancer Prevention Research

JF - Cancer Prevention Research

SN - 1940-6207

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