Haplotype diversity and population structure in cultivated and wild barley evaluated for Fusarium head blight responses

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Abstract

Fusarium head blight (FHB) is a threat to barley (Hordeum vulgare L.) production in many parts of the world. A number of barley accessions with partial resistance have been reported and used in mapping experiments to identify quantitative trait loci (QTL) associated with FHB resistance. Here, we present a set of barley germplasm that exhibits FHB resistance identified through screening a global collection of 23,255 wild (Hordeum vulgare ssp. spontaneum) and cultivated (Hordeum vulgare ssp. vulgare) accessions. Seventy-eight accessions were classified as resistant or moderately resistant. The collection of FHB resistant accessions consists of 5, 27, 46 of winter, wild and spring barley, respectively. The population structure and genetic relationships of the germplasm were investigated with 1,727 Diversity Array Technology (DArT) markers. Multiple clustering analyses suggest the presence of four subpopulations. Within cultivated barley, substructure is largely centered on spike morphology and growth habit. Analysis of molecular variance indicated highly significant genetic variance among clusters and within clusters, suggesting that the FHB resistant sources have broad genetic diversity. The haplotype diversity was characterized with DArT markers associated with the four FHB QTLs on chromosome 2H bin8, 10 and 13 and 6H bin7. In general, the wild barley accessions had distinct haplotypes from those of cultivated barley. The haplotype of the resistant source Chevron was the most prevalent in all four QTL regions, followed by those of the resistant sources Fredrickson and CIho4196. These resistant QTL haplotypes were rare in the susceptible cultivars and accessions grown in the upper Midwest USA. Some two- and six-rowed accessions were identified with high FHB resistance, but contained distinct haplotypes at FHB QTLs from known resistance sources. These germplasm warrant further genetic studies and possible incorporation into barley breeding programs.

Original languageEnglish (US)
Pages (from-to)619-636
Number of pages18
JournalTheoretical and Applied Genetics
Volume126
Issue number3
DOIs
StatePublished - Mar 2013

Bibliographical note

Funding Information:
This work was supported by grants from the U.S. Wheat and Barley Scab Initiative (United States Department of Agriculture-Agricultural Research Service) to G. J. Muehlbauer, K. P. Smith and B. J. Steffenson, grant from Minnesota Small Grains Initiative to G. J. Muehlbauer, and Lieberman-Okinow Endowment at the University of Minnesota to B. J. Steffenson. We thank Harold Bockelman (USDA-ARS National Small Grains Collection); O. Kovaleva and I. Loskotov (N. I. Vavilov All-Russian Scientific Research Institute of Plant Industry); G. Kleijer (Station federale de recherché en production vegetale de Changins); F. Ottosson (Nordic Gene Bank, now NordGen); Y. Anikster (Institute for Cereal Crops Improvement); J. Valkoun (International Center for Agricultural Research in the Dry Areas); and D. Kessler and R. St. Pierre (Plant Genetic Resources of Canada) for providing the germplasm used in this study.

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