Gut colonization of healthy children and their mothers with pathogenic ciprofloxacin-resistant Escherichia coli

Emily A. Gurnee, I. Malick Ndao, James R. Johnson, Brian D. Johnston, Mark D. Gonzalez, Carey Ann D. Burnham, Carla M. Hall-Moore, Jessica E. McGhee, Alexander Mellmann, Barbara B. Warner, Phillip I. Tarr

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

Background. The reservoir of pathogenic ciprofloxacin-resistant Escherichia coli remains unknown. Methods. We conducted a prospective cohort study of 80 healthy twins and their mothers to determine the frequency of excretion of ciprofloxacin-resistant, potentially pathogenic E. coli. Stool specimens were cultured selectively for ciprofloxacin-resistant gram-negative bacteria. Isolates were categorized on the basis of additional resistance and virulence profiles. We also prospectively collected clinical metadata. Results. Fifteen children (19%) and 8 mothers (20%) excreted ciprofloxacin-resistant E. coli at least once. Overall, 33% of 40 families had at least 1 member whose stool specimen yielded ciprofloxacin-resistant E. coli on culture. Fifty-seven submitted stool specimens (2.8%) contained such organisms; clones ST131-H30 and ST405 accounted for 52 and 5 of the positive specimens, respectively. Length of hospital stay after birth (P =.002) and maternal colonization (P =.0001) were associated with subsequent childhood carriage of ciprofloxacin-resistant E. coli; antibiotic use, acid suppression, sex, mode of delivery, and maternal perinatal antibiotic use were not. Ciprofloxacin-resistant E. coli were usually resistant to additional antibiotic classes, and all had virulence genotypes typical of extraintestinal pathogenic E. coli. Conclusions. Healthy children and their mothers commonly harbor ciprofloxacin-resistant E. coli with pathogenic potential.

Original languageEnglish (US)
Pages (from-to)1862-1868
Number of pages7
JournalJournal of Infectious Diseases
Volume212
Issue number12
DOIs
StatePublished - 2015

Keywords

  • Ciprofloxacin-resistant E. coli
  • E. coli ST131-H30
  • E. coli ST405
  • Extra-intestinal pathogenic E. coli
  • Urinary tract infections

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    Gurnee, E. A., Ndao, I. M., Johnson, J. R., Johnston, B. D., Gonzalez, M. D., Burnham, C. A. D., Hall-Moore, C. M., McGhee, J. E., Mellmann, A., Warner, B. B., & Tarr, P. I. (2015). Gut colonization of healthy children and their mothers with pathogenic ciprofloxacin-resistant Escherichia coli. Journal of Infectious Diseases, 212(12), 1862-1868. https://doi.org/10.1093/infdis/jiv278