Guiding synchrotron X-ray diffraction by multimodal video-rate protein crystal imaging

Justin A. Newman, Shijie Zhang, Shane Z. Sullivan, Ximeng Y. Dow, Michael Becker, Michael J. Sheedlo, Sergey Stepanov, Mark S. Carlsen, R. Michael Everly, Chittaranjan Das, Robert F. Fischetti, Garth J. Simpson

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Synchronous digitization, in which an optical sensor is probed synchronously with the firing of an ultrafast laser, was integrated into an optical imaging station for macromolecular crystal positioning prior to synchrotron X-ray diffraction. Using the synchronous digitization instrument, second-harmonic generation, two-photon-excited fluorescence and bright field by laser transmittance were all acquired simultaneously with perfect image registry at up to video-rate (15 frames s-1). A simple change in the incident wavelength enabled simultaneous imaging by two-photon-excited ultraviolet fluorescence, one-photon-excited visible fluorescence and laser transmittance. Development of an analytical model for the signal-to-noise enhancement afforded by synchronous digitization suggests a 15.6-fold improvement over previous photon-counting techniques. This improvement in turn allowed acquisition on nearly an order of magnitude more pixels than the preceding generation of instrumentation and reductions of well over an order of magnitude in image acquisition times. These improvements have allowed detection of protein crystals on the order of 1 μm in thickness under cryogenic conditions in the beamline. These capabilities are well suited to support serial crystallography of crystals approaching 1 μm or less in dimension.Synchronous digitization, in which an optical sensor is probed synchronously with the firing of an ultrafast laser, was integrated into an optical imaging station for macromolecular crystal positioning prior to synchrotron X-ray diffraction. Using the synchronous digitization instrument, second-harmonic generation, two-photon-excited fluorescence, one-photon-excited fluorescence, two-photon-excited ultraviolet fluorescence and bright field by laser transmittance were all acquired with perfect image registry at up to video rate (15 frames s-1).

Original languageEnglish (US)
Pages (from-to)959-965
Number of pages7
JournalJournal of Synchrotron Radiation
Volume23
Issue number4
DOIs
StatePublished - Jul 1 2016
Externally publishedYes

Bibliographical note

Publisher Copyright:
© International Union of Crystallography, 2016.

Keywords

  • nonlinear optical microscopy
  • protein crystal
  • synchronous digitization

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