Vascular smooth muscle cells (VSMCs) play important roles in cardiovascular disorders and biology. Outlined in this paper is a step-by-step procedure for isolating aortic VSMCs from adult C57BL6J male mice by enzymatic digestion of the aorta using collagenase. The plating, culturing, and subculturing of the isolated cells are discussed in detail along with techniques to characterize VSMC phenotype by gene expression and immunofluorescence. Traction force microscopy was used to characterize contractility of single subcultured VSMCs at baseline.
Bibliographical noteFunding Information:
The sources of funding are from DK54733 (LW), DK60521 (LW), LHI Innovation Grant (LW, JLH), and the Lindahl Foundation (JLH).
© 2015, Springer Science+Business Media New York.
- Calponin 1
- Smooth muscle 22α
- Smooth muscle myosin heavy chain
- Smooth muscle α-actin
- Vascular smooth muscle cell