An aligned engineered microvascular network is critical to the culture of thick or highly metabolic tissue in vitro due to the need for inlet and outlet sides for perfusion of the network. Contact guidance may be a way to achieve aligned networks, but the relationship between the alignment of endothelial sprouts and the alignment of extracellular matrix fibers has yet to be fully elucidated. The data presented here show that sprouts from human blood outgrowth endothelial cell spheroids align with fibrin fibrils, and that the extent to which the sprouts align depends upon the strength of the fibril alignment. This was true for both magnetically-aligned fibrin and fibrin aligned via cell-induced gel compaction, although magnetically-aligned fibrin was more effective over the same culture period. The data also demonstrate that longer sprouts are grown when the fibrils, and thus the sprouts, are more strongly aligned. The formation of aligned endothelial sprouts using these methods can be an essential step in the generation of aligned microvascular networks.
|Original language||English (US)|
|Number of pages||8|
|State||Published - Sep 2011|
Bibliographical noteFunding Information:
The authors thank Dr. Xiaoping Wu, Dr. Julien Sein and others at the Center for Magnetic Resonance Imaging for use of the MRI equipment and the Institution for Engineering in Medicine at the University of Minnesota for funding. They also thank Dr. Robert Hebbel at the University of Minnesota for the hBOECs and Paul Carlson and Emmelene Yuan for technical assistance.
Copyright 2012 Elsevier B.V., All rights reserved.
- Endothelial cell