GTP (γS) and GDP (βS) as electron donors: New wine in old bottles

Douglas A Peterson; Daniel C. Peterson; Helen L. Reeve; Stephen L. Archer; Edward K Weir

doi:10.1016/S0024-3205(99)00347-1

GTP (γS) and GDP (βS) as electron donors: New wine in old bottles

Douglas A Peterson, Daniel C. Peterson, Helen L. Reeve, Stephen L. Archer, Edward K Weir

Medicine - Veteran's Administration Medical Center

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

G proteins are membrane-bound regulatory proteins which modulate the activity of ion channels and other effector systems. The GTP and GDP analogs GTP (γS) and GDP (βS) have been used to study the role of G proteins in numerous physiologic systems. The prolonged effects of these analogs have been thought to be due to the fact that they are nonhydrolyzable. However, in this paper we show that the GTP (γS) and GDP (βS) analogs are potent reducing agents at physiologic pH. This observation suggests that previous data obtained using these compounds may need to be reinterpreted.

Original language	English (US)
Pages (from-to)	1135-1140
Number of pages	6
Journal	Life Sciences
Volume	65
Issue number	11
DOIs	https://doi.org/10.1016/S0024-3205(99)00347-1
State	Published - Aug 6 1999

Bibliographical note

Funding Information:
This work was supported by VA Merit Review Research Funding of E. Kenneth Weir.

Keywords

G proteins
GDP (βS)
GTP (γS)
Ion channels
Receptors
Redox

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10.1016/S0024-3205(99)00347-1

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abstract = "G proteins are membrane-bound regulatory proteins which modulate the activity of ion channels and other effector systems. The GTP and GDP analogs GTP (γS) and GDP (βS) have been used to study the role of G proteins in numerous physiologic systems. The prolonged effects of these analogs have been thought to be due to the fact that they are nonhydrolyzable. However, in this paper we show that the GTP (γS) and GDP (βS) analogs are potent reducing agents at physiologic pH. This observation suggests that previous data obtained using these compounds may need to be reinterpreted.",

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T1 - GTP (γS) and GDP (βS) as electron donors

T2 - New wine in old bottles

AU - Peterson, Douglas A

AU - Peterson, Daniel C.

AU - Reeve, Helen L.

AU - Archer, Stephen L.

AU - Weir, Edward K

N1 - Funding Information: This work was supported by VA Merit Review Research Funding of E. Kenneth Weir.

PY - 1999/8/6

Y1 - 1999/8/6

N2 - G proteins are membrane-bound regulatory proteins which modulate the activity of ion channels and other effector systems. The GTP and GDP analogs GTP (γS) and GDP (βS) have been used to study the role of G proteins in numerous physiologic systems. The prolonged effects of these analogs have been thought to be due to the fact that they are nonhydrolyzable. However, in this paper we show that the GTP (γS) and GDP (βS) analogs are potent reducing agents at physiologic pH. This observation suggests that previous data obtained using these compounds may need to be reinterpreted.

AB - G proteins are membrane-bound regulatory proteins which modulate the activity of ion channels and other effector systems. The GTP and GDP analogs GTP (γS) and GDP (βS) have been used to study the role of G proteins in numerous physiologic systems. The prolonged effects of these analogs have been thought to be due to the fact that they are nonhydrolyzable. However, in this paper we show that the GTP (γS) and GDP (βS) analogs are potent reducing agents at physiologic pH. This observation suggests that previous data obtained using these compounds may need to be reinterpreted.

KW - G proteins

KW - GDP (βS)

KW - GTP (γS)

KW - Ion channels

KW - Receptors

KW - Redox

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ER -

GTP (γS) and GDP (βS) as electron donors: New wine in old bottles

Abstract

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Keywords

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