Growth of lepidopteran epithelial cells and hemocytes in primary cultures

T. J. Kurtti, Marion A. Brooks

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Cells from four species of Lepidoptera larvae known to be susceptible to several intracellular pathogens were cultured in vitro. Undifferentiated cells had the greatest potential for growth and survival. Imaginal wing disc explants formed extensive monolayers as a result of cell migration and mitosis. Mitoses were abundant in prohemocytes but not in other types of hemocytes. Differentiated larval tissues were unsuitable sources of cells for continuous culture. Migration of cells from these tissue explants was limited, and those cells which isolated themselves from the explant did not divide.

Original languageEnglish (US)
Pages (from-to)341-350
Number of pages10
JournalJournal of Invertebrate Pathology
Volume15
Issue number3
DOIs
StatePublished - May 1970

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