Growth hormone is permissive for neoplastic colon growth

Vera Chesnokova, Svetlana Zonis, Cuiqi Zhou, Maria Victoria Recouvreux, Anat Ben-Shlomo, Takako Araki, Robert Barrett, Michael Workman, Kolja Wawrowsky, Vladimir A. Ljubimov, Magdalena Uhart, Shlomo Melmed

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Growth hormone (GH) excess in acromegaly is associated with increased precancerous colon polyps and soft tissue adenomas, whereas short-stature humans harboring an inactivating GH receptor mutation do not develop cancer. We show that locally expressed colon GH is abundant in conditions predisposing to colon cancer and in colon adenocarcinoma-associated stromal fibroblasts. Administration of a GH receptor (GHR) blocker in acromegaly patients induced colon p53 and adenomatous polyposis coli (APC), reversing progrowth GH signals. p53 was also induced in skin fibroblasts derived from short-statured humans with mutant GHR. GH-deficient prophet of pituitary-specific positive transcription factor 1 (Prop1)-/- mice exhibited induced colon p53 levels, and cross-breeding them with Apcmin+/- mice that normally develop intestinal and colon tumors resulted in GH-deficient double mutants with markedly decreased tumor number and size. We also demonstrate that GH suppresses p53 and reduces apoptosis in human colon cell lines as well as in induced human pluripotent stem cell-derived intestinal organoids, and confirm in vivo that GH suppresses colon mucosal p53/p21. GH excess leads to decreased colon cell phosphatase and tensin homolog deleted on chromosome 10 (PTEN), increased cell survival with down-regulated APC, nuclear β-catenin accumulation, and increased epithelial-mesenchymal transition factors and colon cell motility.We propose that GH is a molecular component of the "field change" milieu permissive for neoplastic colon growth.

Original languageEnglish (US)
Pages (from-to)E3250-E3259
JournalProceedings of the National Academy of Sciences of the United States of America
Volume113
Issue number23
DOIs
StatePublished - Jun 7 2016

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Growth Hormone
Colon
Growth
Somatotropin Receptors
Adenomatous Polyposis Coli
Acromegaly
Colonic Neoplasms
Fibroblasts
Organoids
Catenins
Induced Pluripotent Stem Cells
Neoplasms
Chromosomes, Human, Pair 10
Epithelial-Mesenchymal Transition
Polyps
Phosphoric Monoester Hydrolases
Adenoma
Cell Movement
Breeding
Cell Survival

Keywords

  • Acromegaly
  • Colon
  • Growth hormone
  • Growth hormone deficiency

Cite this

Growth hormone is permissive for neoplastic colon growth. / Chesnokova, Vera; Zonis, Svetlana; Zhou, Cuiqi; Recouvreux, Maria Victoria; Ben-Shlomo, Anat; Araki, Takako; Barrett, Robert; Workman, Michael; Wawrowsky, Kolja; Ljubimov, Vladimir A.; Uhart, Magdalena; Melmed, Shlomo.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 113, No. 23, 07.06.2016, p. E3250-E3259.

Research output: Contribution to journalArticle

Chesnokova, V, Zonis, S, Zhou, C, Recouvreux, MV, Ben-Shlomo, A, Araki, T, Barrett, R, Workman, M, Wawrowsky, K, Ljubimov, VA, Uhart, M & Melmed, S 2016, 'Growth hormone is permissive for neoplastic colon growth', Proceedings of the National Academy of Sciences of the United States of America, vol. 113, no. 23, pp. E3250-E3259. https://doi.org/10.1073/pnas.1600561113
Chesnokova, Vera ; Zonis, Svetlana ; Zhou, Cuiqi ; Recouvreux, Maria Victoria ; Ben-Shlomo, Anat ; Araki, Takako ; Barrett, Robert ; Workman, Michael ; Wawrowsky, Kolja ; Ljubimov, Vladimir A. ; Uhart, Magdalena ; Melmed, Shlomo. / Growth hormone is permissive for neoplastic colon growth. In: Proceedings of the National Academy of Sciences of the United States of America. 2016 ; Vol. 113, No. 23. pp. E3250-E3259.
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AU - Barrett, Robert

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