Growth enhancement and food conversion efficiency of transgenic fish Labeo rohita

Thayanithy Venugopal, Vikas Anathy, Santhakumar Kirankumar, Thavamani Jegajothivel Pandian

Research output: Contribution to journalArticlepeer-review

43 Scopus citations

Abstract

Three family lines of fast growing transgenic rohu Labeo rohita (rohu) were generated by electroporated-sperm-mediated transfer of the vectors harboring CMV promoter or grass carp β-actin promoter fused to endogenous rohu GH (rGH) cDNA. The gene transfer efficiency was 25%. The transgenic rohu (family line 1) with CMV promoter showed a growth enhancement of four times normal size, whereas those (family lines 2 and 3) generated with β-actin promoter grew 4.5 and 5.8 times faster than their respective control siblings. Southern analysis confirmed the transgene extrachromosomal (Te) persistence until the 60th week in family 1. The individuals of family lines 2 and 3, however, showed integration (Ti), as well as persistence as extarchromosomal copies (Te) until the age of 30 weeks. Mosaicism of the transgene was shown at the levels of its presence and expression. The ectopic expression of rGH mRNA was confirmed by RT-PCR. Feeding experiments revealed that the transgenic rohu ate food at a lower rate but grew more efficiently than their control siblings.

Original languageEnglish (US)
Pages (from-to)477-490
Number of pages14
JournalJournal of Experimental Zoology Part A: Comparative Experimental Biology
Volume301
Issue number6
DOIs
StatePublished - Jun 1 2004

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