Myoblasts from pupae of three species of Lepidoptera were cultured in the Wyatt-Grace medium. Clusters of myoblasts within a heterogeneous monolayer of cells (obtained from the developing head) grew and differentiated in vitro. Extensive networks of myotubes surrounded by proliferating myoblasts were formed. Myotubes became striated by the third day and contractile by the sixth day. Myoblasts isolated from thoracic dorso-longitudinal muscle anlage and cultured as a homogeneous population of cells failed to grow and differentiate.
Bibliographical noteFunding Information:
The work was supported in part by USPHS research grant no. AI 00961f, rom the National Institute of Allergy and Infectious Diseases of the National Instituteso f Health.