Growth and differentiation of lepidopteran myoblasts in vitro

Timothy J Kurtti, Marion A. Brooks

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Myoblasts from pupae of three species of Lepidoptera were cultured in the Wyatt-Grace medium. Clusters of myoblasts within a heterogeneous monolayer of cells (obtained from the developing head) grew and differentiated in vitro. Extensive networks of myotubes surrounded by proliferating myoblasts were formed. Myotubes became striated by the third day and contractile by the sixth day. Myoblasts isolated from thoracic dorso-longitudinal muscle anlage and cultured as a homogeneous population of cells failed to grow and differentiate.

Original languageEnglish (US)
Pages (from-to)407-412
Number of pages6
JournalExperimental Cell Research
Volume61
Issue number2-3
DOIs
StatePublished - Aug 1970

Bibliographical note

Funding Information:
The work was supported in part by USPHS research grant no. AI 00961f, rom the National Institute of Allergy and Infectious Diseases of the National Instituteso f Health.

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