Growth and analysis of crystal forms of toxic shock syndrome toxin 1

Cathleen A. Earhart, G. Sridhar Prasad, Debra L. Murray, Richard P. Novick, Patrick M. Schlievert, Douglas H Ohlendorf

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Native toxic shock syndrom toxin 1 (TSST‐1) purified from Staphylococcus aurius has been crystallized in four different forms. The highest resolution data (2.05 Å) was collected from orthorhombic crystals belonging to the space group C2221. The unit cell dimension are a = 108.7 Å, b = 177.5 Å, c = 97.6 Å. Rotation function analysis of this from indicates that there is trimer of toxin molecules in the asymmetric unit with a local 3‐fold axis parallel to the crystallographic c axis. Crystals of a double mutant of TSST‐1 have been grown which has a single molecule in the asymmetric unit and diffract to 1.9 Å. The space group is P21 with unit cell parameters of a = 44.4 Å, b = 34.0 Å, c = 55.2 Å, β = 93.0°. © 1993 Wiley‐Liss, Inc.

Original languageEnglish (US)
Pages (from-to)329-334
Number of pages6
JournalProteins: Structure, Function, and Bioinformatics
Issue number3
StatePublished - Nov 1993


  • Staphylococcus aureus
  • X‐ray crystallography
  • bacterial toxins
  • crystallization
  • superantigens


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