Methods for propagating murine B-cell precursors in vitro have been instrumental in unraveling the cells and molecules regulating murine B-cell ontogeny. However, similar methods are not available for studying human B-cell ontogeny. Here, Tucker LeBien discusses why efforts to grow human B-cell precursors in vitro have been largely unsuccessful and proposes alternative strategies that may prove useful.
Bibliographical noteFunding Information:
I thank Lewis Steinberg, Judy Villablanca, Martin Wolf, and two anonymous reviewers for their helpful comments and Lisa Hetletvedt for preparation of the manuscript. Tucker W. LeBien is supported by grants CA-21737 and CA-31685 from the National Cancer Institute, a scholar award from the Leukemia Society of America and the Leukemia Task Force.