Growing Chlorella vulgaris on thermophilic anaerobic digestion swine manure for nutrient removal and biomass production

Xiang Yuan Deng, Kun Gao, Ren Chuan Zhang, Min M Addy, Qian Lu, Hong Yan Ren, Paul L Chen, Yu Huan Liu, R. R Ruan

Research output: Contribution to journalArticlepeer-review

62 Scopus citations


Liquid swine manure was subjected to thermophilic anaerobic digestion, ammonia stripping and centrifugation in order to increase the available carbon sources and decrease the ammonia concentration and turbidity. Chlorella vulgaris (UTEX 2714) was grown on minimally diluted (2×, 3× and 4×) autoclaved and non-autoclaved pretreated anaerobic digestion swine manure (PADSM) in a batch-culture system for 7 days. Results showed that C. vulgaris (UTEX 2714) grew best on 3× PADSM media, and effectively removed NH4+-N, TN, TP and COD by 98.5–99.8%, 49.2–55.4%, 20.0–29.7%, 31.2–34.0% and 99.8–99.9%, 67.4–70.8%, 49.3–54.4%, 73.6–78.7% in differently diluted autoclaved and non-autoclaved PADSM, respectively. Results of chemical compositions indicated that contents of pigment, carbohydrate, protein and lipid in C. vulgaris (UTEX 2714) changed with the culture conditions. Moreover, its fatty acid profiles suggested that this alga could be used as animal feed if cultivated in autoclaved PADSM or as good-quality biodiesel feedstock if cultivated in non-autoclaved PADSM.

Original languageEnglish (US)
Pages (from-to)417-425
Number of pages9
JournalBioresource Technology
StatePublished - 2017

Bibliographical note

Funding Information:
This manuscript was supported in part by the Jiangsu Overseas Research and Training Program for University Prominent Young and Middle-aged Teachers and Presidents, the Jiangsu Provincial Natural Science Foundation of China (no. BY2015065-10), and the Minnesota Environment and Natural Resources Trust Fund as recommended by the Legislative Citizen Commission on Minnesota Resources (LCCMR) and University of Minnesota Center for Biorefining.

Publisher Copyright:
© 2017 Elsevier Ltd


  • Chemical composition
  • Chlorella vulgaris
  • Fatty acid profiles
  • Nutrient removal
  • Swine manure


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