Graphene oxide as a matrix for enzyme immobilization

Jingyan Zhang, Jiali Zhang, Feng Zhang, Haijun Yang, Xuelei Huang, Hui Liu, Shouwu Guo

Research output: Contribution to journalArticlepeer-review

502 Scopus citations


Graphene oxide (GO), having a large specific surface area and abundant functional groups, provides an ideal substrate for study enzyme immobilization. We demonstrated that the enzyme immobilization on the GO sheets could take place readily without using any cross-linking reagents and additional surface modification. The atomically flat surface enabled us to observe the immobilized enzyme in the native state directly using atomic force microscopy (AFM). Combining the AFM imaging results of the immobilized enzyme molecules and their catalytic activity, we illustrated that the conformation of the immobilized enzyme is mainly determined by interactions of enzyme molecules with the functional groups of GO.

Original languageEnglish (US)
Pages (from-to)6083-6085
Number of pages3
Issue number9
StatePublished - May 4 2010
Externally publishedYes


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