Gonadal steroid modulation of basal and vasoactive intestinal polypeptide-stimulated prolactin release by Turkey anterior pituitary cells

T. R. Knapp; S. C. Fehrer; J. L. Silsby; T. E. Porter; E. J. Behnke; M. E. El Halawani

doi:10.1016/0016-6480(88)90205-5

Gonadal steroid modulation of basal and vasoactive intestinal polypeptide-stimulated prolactin release by Turkey anterior pituitary cells

T. R. Knapp, S. C. Fehrer, J. L. Silsby, T. E. Porter, E. J. Behnke, M. E. El Halawani

Animal Science

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Porcine vasoactive intestinal polypeptide (VIP; 10^-9 to 10^-7 M) was a potent stimulator of prolactin (PRL) release by anterior pituitary cells from immature and laying turkey hens. Basal and VIP-induced PRL release of cells from laying hens were diminished (P < 0.05) when the cells were cultured for 48 hr in the presence of charcoal-stripped laying hen serum, but not when the cells were cultured in the presence of whole laying hen serum. This change in VIP-induced PRL release was not evident when cells were derived from immature hens. Basal PRL release by cells from laying hens was not altered by the presence of estradiol (E₂; 10^-12 to 10^-5 M), although such release was generally enhanced in cultures of cells from immature hens containing E₂. The presence of E₂ enhanced (P < 0.05) the magnitude of the VIP-induced PRL release by cultures of cells from laying hens and diminished (P < 0.05) the magnitude of this release in cultures of cells from immature hens. Cells from immature and laying hens exposed to progesterone (P₄; 10^-5 M) for 96 hr exhibited enhanced basal PRL release, though lower P₄ concentrations had no effect. Utilizing cells from laying hens, P₄ exposure for 24 hr resulted in diminished (P < 0.05) VIP-induced PRL release, while P₄ exposure for 96 hr resulted in markedly enhanced (P < 0.05) VIP-induced PRL release. Basal PRL release was generally not altered by the presence of testosterone (T). The VIP-induced PRL release by cells derived from immature and laying hens was diminished (P < 0.05) by the presence of T. Prolactin release in the turkey is likely modulated by gonadal steroids acting directly on the cells of the anterior pituitary.

Original language	English (US)
Pages (from-to)	226-236
Number of pages	11
Journal	General and Comparative Endocrinology
Volume	72
Issue number	2
DOIs	https://doi.org/10.1016/0016-6480(88)90205-5
State	Published - Nov 1988

Bibliographical note

Funding Information:
i This work was supported by USDA Grant 85 CRCR-1-1838. This is Scientific Journal Series paper 16,177 of the Minnesota Agricultural Experiment Station.

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title = "Gonadal steroid modulation of basal and vasoactive intestinal polypeptide-stimulated prolactin release by Turkey anterior pituitary cells",

abstract = "Porcine vasoactive intestinal polypeptide (VIP; 10-9 to 10-7 M) was a potent stimulator of prolactin (PRL) release by anterior pituitary cells from immature and laying turkey hens. Basal and VIP-induced PRL release of cells from laying hens were diminished (P < 0.05) when the cells were cultured for 48 hr in the presence of charcoal-stripped laying hen serum, but not when the cells were cultured in the presence of whole laying hen serum. This change in VIP-induced PRL release was not evident when cells were derived from immature hens. Basal PRL release by cells from laying hens was not altered by the presence of estradiol (E2; 10-12 to 10-5 M), although such release was generally enhanced in cultures of cells from immature hens containing E2. The presence of E2 enhanced (P < 0.05) the magnitude of the VIP-induced PRL release by cultures of cells from laying hens and diminished (P < 0.05) the magnitude of this release in cultures of cells from immature hens. Cells from immature and laying hens exposed to progesterone (P4; 10-5 M) for 96 hr exhibited enhanced basal PRL release, though lower P4 concentrations had no effect. Utilizing cells from laying hens, P4 exposure for 24 hr resulted in diminished (P < 0.05) VIP-induced PRL release, while P4 exposure for 96 hr resulted in markedly enhanced (P < 0.05) VIP-induced PRL release. Basal PRL release was generally not altered by the presence of testosterone (T). The VIP-induced PRL release by cells derived from immature and laying hens was diminished (P < 0.05) by the presence of T. Prolactin release in the turkey is likely modulated by gonadal steroids acting directly on the cells of the anterior pituitary.",

author = "Knapp, {T. R.} and Fehrer, {S. C.} and Silsby, {J. L.} and Porter, {T. E.} and Behnke, {E. J.} and {El Halawani}, {M. E.}",

note = "Funding Information: i This work was supported by USDA Grant 85 CRCR-1-1838. This is Scientific Journal Series paper 16,177 of the Minnesota Agricultural Experiment Station.",

year = "1988",

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AU - Knapp, T. R.

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AU - Silsby, J. L.

AU - Porter, T. E.

AU - Behnke, E. J.

AU - El Halawani, M. E.

N1 - Funding Information: i This work was supported by USDA Grant 85 CRCR-1-1838. This is Scientific Journal Series paper 16,177 of the Minnesota Agricultural Experiment Station.

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N2 - Porcine vasoactive intestinal polypeptide (VIP; 10-9 to 10-7 M) was a potent stimulator of prolactin (PRL) release by anterior pituitary cells from immature and laying turkey hens. Basal and VIP-induced PRL release of cells from laying hens were diminished (P < 0.05) when the cells were cultured for 48 hr in the presence of charcoal-stripped laying hen serum, but not when the cells were cultured in the presence of whole laying hen serum. This change in VIP-induced PRL release was not evident when cells were derived from immature hens. Basal PRL release by cells from laying hens was not altered by the presence of estradiol (E2; 10-12 to 10-5 M), although such release was generally enhanced in cultures of cells from immature hens containing E2. The presence of E2 enhanced (P < 0.05) the magnitude of the VIP-induced PRL release by cultures of cells from laying hens and diminished (P < 0.05) the magnitude of this release in cultures of cells from immature hens. Cells from immature and laying hens exposed to progesterone (P4; 10-5 M) for 96 hr exhibited enhanced basal PRL release, though lower P4 concentrations had no effect. Utilizing cells from laying hens, P4 exposure for 24 hr resulted in diminished (P < 0.05) VIP-induced PRL release, while P4 exposure for 96 hr resulted in markedly enhanced (P < 0.05) VIP-induced PRL release. Basal PRL release was generally not altered by the presence of testosterone (T). The VIP-induced PRL release by cells derived from immature and laying hens was diminished (P < 0.05) by the presence of T. Prolactin release in the turkey is likely modulated by gonadal steroids acting directly on the cells of the anterior pituitary.

AB - Porcine vasoactive intestinal polypeptide (VIP; 10-9 to 10-7 M) was a potent stimulator of prolactin (PRL) release by anterior pituitary cells from immature and laying turkey hens. Basal and VIP-induced PRL release of cells from laying hens were diminished (P < 0.05) when the cells were cultured for 48 hr in the presence of charcoal-stripped laying hen serum, but not when the cells were cultured in the presence of whole laying hen serum. This change in VIP-induced PRL release was not evident when cells were derived from immature hens. Basal PRL release by cells from laying hens was not altered by the presence of estradiol (E2; 10-12 to 10-5 M), although such release was generally enhanced in cultures of cells from immature hens containing E2. The presence of E2 enhanced (P < 0.05) the magnitude of the VIP-induced PRL release by cultures of cells from laying hens and diminished (P < 0.05) the magnitude of this release in cultures of cells from immature hens. Cells from immature and laying hens exposed to progesterone (P4; 10-5 M) for 96 hr exhibited enhanced basal PRL release, though lower P4 concentrations had no effect. Utilizing cells from laying hens, P4 exposure for 24 hr resulted in diminished (P < 0.05) VIP-induced PRL release, while P4 exposure for 96 hr resulted in markedly enhanced (P < 0.05) VIP-induced PRL release. Basal PRL release was generally not altered by the presence of testosterone (T). The VIP-induced PRL release by cells derived from immature and laying hens was diminished (P < 0.05) by the presence of T. Prolactin release in the turkey is likely modulated by gonadal steroids acting directly on the cells of the anterior pituitary.

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Gonadal steroid modulation of basal and vasoactive intestinal polypeptide-stimulated prolactin release by Turkey anterior pituitary cells

Abstract

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