Gonadal steroid-mediated alteration of luteinizing hormone secretion by anterior pituitary cells of young turkeys

T. R. Knapp; S. C. Fehrer; J. L. Silsby; T. E. Porter; E. J. Behnke; M. E. El Halawani

doi:10.1016/0016-6480(87)90084-0

Gonadal steroid-mediated alteration of luteinizing hormone secretion by anterior pituitary cells of young turkeys

T. R. Knapp
, S. C. Fehrer
, J. L. Silsby
, T. E. Porter
, E. J. Behnke
, M. E. El Halawani

Animal Science

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

The magnitude of luteinizing hormone (LH) release during a 3-hr test incubation was diminished (P < 0.05) when anterior pituitary cells from young turkeys were cultured for 24 to 120 hr. This trend was evident with basal LH release and with LH release induced by luteinizing hormone-releasing hormone (LH-RH) or hypothalamic extract. Anterior pituitary cells were cultured with various concentrations (10^-14 to 10^-6 M) of estradiol (E₂), progesterone (P₄), or testosterone (T) for 24 hr and then exposed to LH-RH or control medium for 3 hr, still in the presence of steroids. Basal LH release was potentiated (P < 0.05) when cells were cultured with 10^-8 or 10^-6 M T, but not with E₂ or P₄. When cells were cultured with E₂, LH release in the presence of 10^-8 M LH-RH was enhanced (P < 0.05) in a dose-dependent fashion. LH-RH mediated LH release was also enhanced (P < 0.05) when cells were cultured with 10^-8 M P₄ or 10^-6 M T. Gonadal steroids can act directly on the anterior pituitary of the young domestic turkey to modulate LH release, with T enhancing basal LH release and E₂ potentiating LH-RH-medlated LH release.

Original language	English (US)
Pages (from-to)	449-455
Number of pages	7
Journal	General and Comparative Endocrinology
Volume	68
Issue number	3
DOIs	https://doi.org/10.1016/0016-6480(87)90084-0
State	Published - Dec 1987

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@article{79f1385ad5214806b8e2a00e1fcd2b5b,

title = "Gonadal steroid-mediated alteration of luteinizing hormone secretion by anterior pituitary cells of young turkeys",

abstract = "The magnitude of luteinizing hormone (LH) release during a 3-hr test incubation was diminished (P < 0.05) when anterior pituitary cells from young turkeys were cultured for 24 to 120 hr. This trend was evident with basal LH release and with LH release induced by luteinizing hormone-releasing hormone (LH-RH) or hypothalamic extract. Anterior pituitary cells were cultured with various concentrations (10-14 to 10-6 M) of estradiol (E2), progesterone (P4), or testosterone (T) for 24 hr and then exposed to LH-RH or control medium for 3 hr, still in the presence of steroids. Basal LH release was potentiated (P < 0.05) when cells were cultured with 10-8 or 10-6 M T, but not with E2 or P4. When cells were cultured with E2, LH release in the presence of 10-8 M LH-RH was enhanced (P < 0.05) in a dose-dependent fashion. LH-RH mediated LH release was also enhanced (P < 0.05) when cells were cultured with 10-8 M P4 or 10-6 M T. Gonadal steroids can act directly on the anterior pituitary of the young domestic turkey to modulate LH release, with T enhancing basal LH release and E2 potentiating LH-RH-medlated LH release.",

author = "Knapp, \{T. R.\} and Fehrer, \{S. C.\} and Silsby, \{J. L.\} and Porter, \{T. E.\} and Behnke, \{E. J.\} and \{El Halawani\}, \{M. E.\}",

year = "1987",

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T1 - Gonadal steroid-mediated alteration of luteinizing hormone secretion by anterior pituitary cells of young turkeys

AU - Knapp, T. R.

AU - Fehrer, S. C.

AU - Silsby, J. L.

AU - Porter, T. E.

AU - Behnke, E. J.

AU - El Halawani, M. E.

PY - 1987/12

Y1 - 1987/12

N2 - The magnitude of luteinizing hormone (LH) release during a 3-hr test incubation was diminished (P < 0.05) when anterior pituitary cells from young turkeys were cultured for 24 to 120 hr. This trend was evident with basal LH release and with LH release induced by luteinizing hormone-releasing hormone (LH-RH) or hypothalamic extract. Anterior pituitary cells were cultured with various concentrations (10-14 to 10-6 M) of estradiol (E2), progesterone (P4), or testosterone (T) for 24 hr and then exposed to LH-RH or control medium for 3 hr, still in the presence of steroids. Basal LH release was potentiated (P < 0.05) when cells were cultured with 10-8 or 10-6 M T, but not with E2 or P4. When cells were cultured with E2, LH release in the presence of 10-8 M LH-RH was enhanced (P < 0.05) in a dose-dependent fashion. LH-RH mediated LH release was also enhanced (P < 0.05) when cells were cultured with 10-8 M P4 or 10-6 M T. Gonadal steroids can act directly on the anterior pituitary of the young domestic turkey to modulate LH release, with T enhancing basal LH release and E2 potentiating LH-RH-medlated LH release.

AB - The magnitude of luteinizing hormone (LH) release during a 3-hr test incubation was diminished (P < 0.05) when anterior pituitary cells from young turkeys were cultured for 24 to 120 hr. This trend was evident with basal LH release and with LH release induced by luteinizing hormone-releasing hormone (LH-RH) or hypothalamic extract. Anterior pituitary cells were cultured with various concentrations (10-14 to 10-6 M) of estradiol (E2), progesterone (P4), or testosterone (T) for 24 hr and then exposed to LH-RH or control medium for 3 hr, still in the presence of steroids. Basal LH release was potentiated (P < 0.05) when cells were cultured with 10-8 or 10-6 M T, but not with E2 or P4. When cells were cultured with E2, LH release in the presence of 10-8 M LH-RH was enhanced (P < 0.05) in a dose-dependent fashion. LH-RH mediated LH release was also enhanced (P < 0.05) when cells were cultured with 10-8 M P4 or 10-6 M T. Gonadal steroids can act directly on the anterior pituitary of the young domestic turkey to modulate LH release, with T enhancing basal LH release and E2 potentiating LH-RH-medlated LH release.

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Gonadal steroid-mediated alteration of luteinizing hormone secretion by anterior pituitary cells of young turkeys

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