Gonadal steroid-mediated alteration of luteinizing hormone secretion by anterior pituitary cells of young turkeys

T. R. Knapp; S. C. Fehrer; J. L. Silsby; T. E. Porter; E. J. Behnke; M. E. El Halawani

doi:10.1016/0016-6480(87)90084-0

Gonadal steroid-mediated alteration of luteinizing hormone secretion by anterior pituitary cells of young turkeys

T. R. Knapp, S. C. Fehrer, J. L. Silsby, T. E. Porter, E. J. Behnke, M. E. El Halawani

Animal Science

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Abstract

The magnitude of luteinizing hormone (LH) release during a 3-hr test incubation was diminished (P < 0.05) when anterior pituitary cells from young turkeys were cultured for 24 to 120 hr. This trend was evident with basal LH release and with LH release induced by luteinizing hormone-releasing hormone (LH-RH) or hypothalamic extract. Anterior pituitary cells were cultured with various concentrations (10^-14 to 10^-6 M) of estradiol (E₂), progesterone (P₄), or testosterone (T) for 24 hr and then exposed to LH-RH or control medium for 3 hr, still in the presence of steroids. Basal LH release was potentiated (P < 0.05) when cells were cultured with 10^-8 or 10^-6 M T, but not with E₂ or P₄. When cells were cultured with E₂, LH release in the presence of 10^-8 M LH-RH was enhanced (P < 0.05) in a dose-dependent fashion. LH-RH mediated LH release was also enhanced (P < 0.05) when cells were cultured with 10^-8 M P₄ or 10^-6 M T. Gonadal steroids can act directly on the anterior pituitary of the young domestic turkey to modulate LH release, with T enhancing basal LH release and E₂ potentiating LH-RH-medlated LH release.

Original language	English (US)
Pages (from-to)	449-455
Number of pages	7
Journal	General and Comparative Endocrinology
Volume	68
Issue number	3
DOIs	https://doi.org/10.1016/0016-6480(87)90084-0
State	Published - Dec 1987

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title = "Gonadal steroid-mediated alteration of luteinizing hormone secretion by anterior pituitary cells of young turkeys",

abstract = "The magnitude of luteinizing hormone (LH) release during a 3-hr test incubation was diminished (P < 0.05) when anterior pituitary cells from young turkeys were cultured for 24 to 120 hr. This trend was evident with basal LH release and with LH release induced by luteinizing hormone-releasing hormone (LH-RH) or hypothalamic extract. Anterior pituitary cells were cultured with various concentrations (10-14 to 10-6 M) of estradiol (E2), progesterone (P4), or testosterone (T) for 24 hr and then exposed to LH-RH or control medium for 3 hr, still in the presence of steroids. Basal LH release was potentiated (P < 0.05) when cells were cultured with 10-8 or 10-6 M T, but not with E2 or P4. When cells were cultured with E2, LH release in the presence of 10-8 M LH-RH was enhanced (P < 0.05) in a dose-dependent fashion. LH-RH mediated LH release was also enhanced (P < 0.05) when cells were cultured with 10-8 M P4 or 10-6 M T. Gonadal steroids can act directly on the anterior pituitary of the young domestic turkey to modulate LH release, with T enhancing basal LH release and E2 potentiating LH-RH-medlated LH release.",

author = "Knapp, {T. R.} and Fehrer, {S. C.} and Silsby, {J. L.} and Porter, {T. E.} and Behnke, {E. J.} and {El Halawani}, {M. E.}",

year = "1987",

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T1 - Gonadal steroid-mediated alteration of luteinizing hormone secretion by anterior pituitary cells of young turkeys

AU - Knapp, T. R.

AU - Fehrer, S. C.

AU - Silsby, J. L.

AU - Porter, T. E.

AU - Behnke, E. J.

AU - El Halawani, M. E.

PY - 1987/12

Y1 - 1987/12

N2 - The magnitude of luteinizing hormone (LH) release during a 3-hr test incubation was diminished (P < 0.05) when anterior pituitary cells from young turkeys were cultured for 24 to 120 hr. This trend was evident with basal LH release and with LH release induced by luteinizing hormone-releasing hormone (LH-RH) or hypothalamic extract. Anterior pituitary cells were cultured with various concentrations (10-14 to 10-6 M) of estradiol (E2), progesterone (P4), or testosterone (T) for 24 hr and then exposed to LH-RH or control medium for 3 hr, still in the presence of steroids. Basal LH release was potentiated (P < 0.05) when cells were cultured with 10-8 or 10-6 M T, but not with E2 or P4. When cells were cultured with E2, LH release in the presence of 10-8 M LH-RH was enhanced (P < 0.05) in a dose-dependent fashion. LH-RH mediated LH release was also enhanced (P < 0.05) when cells were cultured with 10-8 M P4 or 10-6 M T. Gonadal steroids can act directly on the anterior pituitary of the young domestic turkey to modulate LH release, with T enhancing basal LH release and E2 potentiating LH-RH-medlated LH release.

AB - The magnitude of luteinizing hormone (LH) release during a 3-hr test incubation was diminished (P < 0.05) when anterior pituitary cells from young turkeys were cultured for 24 to 120 hr. This trend was evident with basal LH release and with LH release induced by luteinizing hormone-releasing hormone (LH-RH) or hypothalamic extract. Anterior pituitary cells were cultured with various concentrations (10-14 to 10-6 M) of estradiol (E2), progesterone (P4), or testosterone (T) for 24 hr and then exposed to LH-RH or control medium for 3 hr, still in the presence of steroids. Basal LH release was potentiated (P < 0.05) when cells were cultured with 10-8 or 10-6 M T, but not with E2 or P4. When cells were cultured with E2, LH release in the presence of 10-8 M LH-RH was enhanced (P < 0.05) in a dose-dependent fashion. LH-RH mediated LH release was also enhanced (P < 0.05) when cells were cultured with 10-8 M P4 or 10-6 M T. Gonadal steroids can act directly on the anterior pituitary of the young domestic turkey to modulate LH release, with T enhancing basal LH release and E2 potentiating LH-RH-medlated LH release.

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Gonadal steroid-mediated alteration of luteinizing hormone secretion by anterior pituitary cells of young turkeys

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