Gonadal steroid-mediated alteration of luteinizing hormone secretion by anterior pituitary cells of young turkeys

T. R. Knapp, S. C. Fehrer, J. L. Silsby, T. E. Porter, E. J. Behnke, M. E. El Halawani

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

The magnitude of luteinizing hormone (LH) release during a 3-hr test incubation was diminished (P < 0.05) when anterior pituitary cells from young turkeys were cultured for 24 to 120 hr. This trend was evident with basal LH release and with LH release induced by luteinizing hormone-releasing hormone (LH-RH) or hypothalamic extract. Anterior pituitary cells were cultured with various concentrations (10-14 to 10-6 M) of estradiol (E2), progesterone (P4), or testosterone (T) for 24 hr and then exposed to LH-RH or control medium for 3 hr, still in the presence of steroids. Basal LH release was potentiated (P < 0.05) when cells were cultured with 10-8 or 10-6 M T, but not with E2 or P4. When cells were cultured with E2, LH release in the presence of 10-8 M LH-RH was enhanced (P < 0.05) in a dose-dependent fashion. LH-RH mediated LH release was also enhanced (P < 0.05) when cells were cultured with 10-8 M P4 or 10-6 M T. Gonadal steroids can act directly on the anterior pituitary of the young domestic turkey to modulate LH release, with T enhancing basal LH release and E2 potentiating LH-RH-medlated LH release.

Original languageEnglish (US)
Pages (from-to)449-455
Number of pages7
JournalGeneral and Comparative Endocrinology
Volume68
Issue number3
DOIs
StatePublished - Dec 1987

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