Going back to the roots: Effective utilisation of HLA typing information for bone marrow registries requires full knowledge of the DNA sequences of the oligonucleotide reagents used in the testing

W. Helmberg, J. Hegland, C. K. Hurley, M. Maiers, S. G.E. Marsh, C. Müller, E. H. Rozemuller

Research output: Contribution to journalComment/debatepeer-review

17 Scopus citations

Abstract

Information obtained by DNA-based HLA typing assays is more detailed and of higher quality than that obtained by conventional serological techniques. Nevertheless, it is common for data acquired in this way to be presented in the more familiar serological format. In many cases this representation can lead to significant loss of information, which may only become apparent at a later time, with the discovery of novel allele sequences. DNA-based typing methods, such as sequence-specific oligonucleotide probing (SSOP) or sequence-specific priming (SSP) generate fragmentary sequence data which is information rich. An alternative to assigning allele names to these fragments is to simply store the sequence data itself without interpretation. Bone marrow donor repositories can then be searched directly with sequence information, which though complex is more complete, rather than searching by derivative allele names.

Original languageEnglish (US)
Pages (from-to)99-102
Number of pages4
JournalTissue Antigens
Volume56
Issue number1
DOIs
StatePublished - 2000

Keywords

  • Bone marrow donor registries
  • HLA
  • SBT
  • SSO
  • SSP
  • Virtual sequence
  • Virtual-DNA

Fingerprint Dive into the research topics of 'Going back to the roots: Effective utilisation of HLA typing information for bone marrow registries requires full knowledge of the DNA sequences of the oligonucleotide reagents used in the testing'. Together they form a unique fingerprint.

Cite this